摘要
从南方红豆杉菌根菌中筛选出产紫杉醇和紫杉烷类化合物10-去乙酰巴卡亭Ⅲ(10-DAB)的高产菌株,对分离获得的21株菌株进行了液体发酵培养实验,通过TLC、HPLC及LC-MS检测发现,有10株菌株具有产紫杉醇及紫杉烷类化合物的能力,其中4株菌株中产10-DAB分别为260.05、92.88、393.27、70.52μg/L,但未检出产紫杉醇;6株中产紫杉醇分别为434.94、1 242.19、200.65、360.77、317.90、29.82μg/L,但未检出产10-DAB,说明南方红豆杉的各种菌根真菌虽具有合成紫杉醇或紫杉烷类化合物的能力,但各自所合成化合物有差异,不同种类的菌根菌可能分别参与了紫杉醇生物合成的不同环节,即南方红豆杉菌根高含量紫杉醇的生物合成可能是多种菌根菌协同参与作用的结果,且其中的高产菌株具有发酵生产紫杉醇的潜力。
To screen high-yield strains which could produce paclitaxel and 10-deacetylbaccation Ⅲ（ 10-DAB） from T. chinensis var. mairei mycorrhizal fungus,21 strains were separated,obtained and carried on liquid fermentation culture. The detection results of TLC,HPLC,and LC-MS show that 10 strains among those possess the ability to produce paclitaxel and taxane,and 10-DAB contents of 4 strains are 260. 05,92. 88,393. 27 and 70. 52 μg / L,respectively. The paclitaxel contents of 6 strains are434. 94,1 242. 19,200. 65,360. 77,317. 90 and 29. 82 μg / L among these 10 strains. These results indicate that the mycorrhizal fungus has the ability to synthesize paxlitaxel and taxanes,but each of the synthesized compounds have the difference. It is the different kinds of mycorrhizal that may participate in the taxol biosynthesis,and high contents of taxol result form collaborative actions. And there is the paclitaxel potential to produce in fermentation culture of the high-yield strains.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2015年第3期114-120,共7页
Chemistry and Industry of Forest Products
基金
湖南省高校产业化项目(2011CY014)
吉首大学校级课题(12Jdy12042)
