摘要
目的探讨中药清肺化痰丸对大气PM2.5致大鼠肺损伤的干预作用。方法于某居住区采集大气PM2.5。健康成年SD雄性大鼠64只随机分为8组:每组8只,分别为空白对照组(不做任何处理)、生理盐水对照组(气管滴注1.5 ml/kg生理盐水)、溶剂对照组(每天灌胃给予10 ml/kg的0.2%羧甲基纤维素,共1周)、干预对照组(每天灌胃给予2.40 g/kg清肺化痰丸,共1周)、染毒对照组(气管滴注40 mg/kg的PM2.5生理盐水混悬液染毒,隔天1次,共3次)以及低、中、高剂量干预组(气管滴注40 mg/kg的PM2.5生理盐水混悬液染毒,隔天1次,共3次,再每天分别灌胃给予0.65、1.20和2.40 g/kg清肺化痰丸,共1周。收集肺泡灌洗液(BALF)进行细胞计数,并测定BALF中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、总蛋白(TP)、白蛋白(ALB)、白介素-1β(IL-1β)、白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平。结果与生理盐水对照组比较,染毒对照组大鼠BALF中ACP、AKP、LDH活力和TP、ALB、IL-6、IL-1β、TNF-α含量及中性粒细胞百分比均升高,差异均有统计学意义(P<0.05)。与染毒对照组比较,各剂量干预组大鼠BALF中ACP、AKP、LDH活力和TP、ALB、IL-6、IL-1β、TNF-α含量及中性粒细胞百分比均降低,差异均有统计学意义(P<0.05),且随干预剂量的升高其降低幅度增加。结论中药清肺化痰丸对气管滴注PM2.5导致的肺损伤有一定减轻作用。
Objective To examine the intervention effect of Qing Fei Hua Tan Wan, a traditional Chinese medicine, on the acute pulmonary injury induced by PM2.5. Methods Ambient PM2.5 was sampled in a residential area for preparation of PM2.5 saline suspension. Sixty-four healthy adult male SD rats were randomly divided into eight groups, eight in each, blank group without any treatment, saline group treated with normal saline of 1.5 ml/kg body weight （BW） by intratracheal instillation, solvent group treated with carboxy methocel through gavage of 10 ml/kg BW, once a day for one week, intervening control group treated with the medicine through gavage （2.40 g/kg BW） for one week. PM2.5-exposure group were exposed by intratracheal instillation of PM2.5 saline suspension with the dose of 40 mg/kg BW, repeated three times for one week. Intervening （low, moderate and high-dose） groups were administered with drug through gavage at the doses of 0.65, 1.20 and 2.40 g/kg BW, respectively, followed by PM2.5-exposure. The collected bronehoalveolar lavage fluid （BALF） was analyzed for the levels of lactate dehydrogenase （LDH）, acid phosphatase （ACP）, alkaline phosphatase （AKP）, total proteins TP）, albumin （ALB）, intedeukin-1βα（IL-1β）, interleukin-6 （IL-6） and necrosis factor alpha （TNF-α. Results The levels of LDH,ACP, AKP, TP,ALB,ILB,IL-6 and TNF-α in the PM2.5-exposure group were higher than those in the saline group. The levels of LDH, ACP, AKP,TP,ALB,IL-1β,IL-6 and TNF-o~ in the intervening groups were lower than those in the PM2.5-exposure group with a significant dose-response correlation（P〈0.05）. Conchlsion Qing Fei Hua Tan Wan may reduce the pulmonary injury induced by PM2.5.
出处
《环境与健康杂志》
CAS
北大核心
2015年第5期377-380,F0003,共5页
Journal of Environment and Health
基金
国家自然科学基金(81172617)
关键词
PM2.5
肺损伤
中药
干预
PM2.5
Pulmonary injury
Chinese medicine
Intervention
