摘要
目的：探讨染料木素对人胚皮肤成纤维细胞（CCC-ESF-1）胶原（collagen, Col）、基质金属蛋白酶（matrix metalloproteinase, MMP）和基质金属蛋白酶组织抑制剂（tissue inhibitors of matrix metalloproteinase, TIMP）mRNA表达的影响。方法体外培养的CCC-ESF-1细胞按随机数字表法分为空白对照组、雌二醇组以及染料木素小、中、大剂量组。采用MTT法检测细胞活力，RT-PCR技术检测ColⅠ、ColⅢ、MMP-1、TIMP-1和TIMP-2 mRNA表达。结果与空白对照组比较，雌二醇组、染料木素中剂量组[（0.451±0.037）、（0.446±0.047）比（0.385±0.061）]均可促进CCC-ESF-1细胞增殖（P均＜0.05），且雌二醇组、染料木素中剂量组细胞 ColⅠ[（0.960±0.012）、（0.929±0.015）比（0.812±0.014）]，Col Ⅲ[（0.892±0.009）、（0.824±0.022）比（0.768±0.025）]、TIMP-1[（0.841±0.023）、（0.838±0.053）比（0.751±0.027）]、TIMP-2[（0.456±0.017）、（0.448±0.036）比（0.381±0.029）]mRNA 水平较空白组增高（P 均＜0.01），MMP-1[（0.398±0.043）、（0.402±0.044）比（0.525±0.006）]mRNA水平较空白组降低（P均＜0.01）。结论染料木素可促进 CCC-ESF-1细胞增殖，可能与上调 ColⅠ、Col Ⅲ、MMP-1、TIMP-1、TIMP-2以及下调MMP-1有关。
Objective To investigate the effects of genistein on the mRNA expressions of collagen （Col）, matrix metalloproteinase （MMP ） and tissue inhibitor of matrix metalloproteinase （TIMP） in human embryonic skin fibroblasts （CCC-ESF-1）.MethodsThe cultured CCC-ESF-1cells were divided into a black control group, an estradiol group and genistein groups of different doses. The mRNA expressions of ColⅠ, ColⅢ , MMP-1, TIMP-1 and TIMP-2 were detected by RT-PCR.Results Compared with the black group, estradiol and medium dose of genistein （0.451 ± 0.037, 0.446 ± 0.047vs.0.385 ± 0.061, allP〈0.05） could promote the proliferation of the CCC-ESF-1 cells, estradiol and medium dose of genistein could up-regulate the mRNA expressions of ColⅠ （0.960 ± 0.012, 0.929 ± 0.015vs.0.812 ± 0.014, allP〈0.01）, ColⅢ （0.892 ± 0.009, 0.824 ± 0.022vs.0.768 ± 0.025, allP〈0.01）, TIMP-1 （0.841 ± 0.023, 0.838 ± 0.053vs.0.751 ± 0.027, allP〈0.01） and TIMP-2 （0.456 ± 0.017, 0.448 ± 0.036vs.0.381 ± 0.029, allP〈0.01）, and down-regulate MMP-1 mRNAexpression （0.398 ± 0.043, 0.402 ± 0.044vs.0.525 ± 0.006, allP〈0.01）.Conclusions Genistein could promote the proliferation of the CCC-ESF-1 cells, and that may be related with up-regulating the mRNA expressions of ColⅠ, ColⅢ , MMP-1, TIMP-1and down-regulating MMP-1 mRNA expression.
出处
《国际中医中药杂志》
2015年第7期617-620,共4页
International Journal of Traditional Chinese Medicine
基金
黑龙江省教育厅科学技术研究项目（12531627）
