摘要
根据Gen Bank公布的小鹅瘟病毒VP1基因保守序列设计了一对引物,建立了检测小鹅瘟病毒PCR方法,并对其特异性、敏感性进行了研究。该PCR方法对小鹅瘟病毒扩增结果为阳性,对照毒株扩增结果均为阴性;对小鹅瘟病毒检测的灵敏性为1pg总DNA量。以上结果表明,该PCR方法特异性强、敏感性高、简便、快速,可用于小鹅瘟的早期确诊和病毒鉴定。
A PCR assay for detection of Gosling plague viruse （GPV）was established using a pair of primers based on the VP1 gene of GPV. and the specificity, sensitivity were studied.This method specifically amplify a fragment from GPV, but not from control virus, with a detection limit of lpg DNA of GPV.Therefore the established PCR technique provided a sensitive, specific,fast and reliablemethod for diagnosis and epizootic study of the GPV
基金
山东省现代产业技术体系家禽创新团队生产与环境控制创新团队项目(SDAIT-13-011-10)
关键词
小鹅瘟病毒
PCR
检测
Gosling plague viruse
PCR
detection
