摘要
目的探讨三七粉(NGP)防治非酒精性脂肪肝病(NAFLD)的作用机制。方法健康雄性昆明种小鼠60只随机分为4组:正常对照组、NAFLD模型组、NGP干预组、瑞舒伐他汀钙(RSTC)干预组,每组15只。通过喂饲高胆固醇饲料、建立NAFLD大鼠模型,采用NGP连续灌胃作用于NAFLD模型小鼠,观察各组小鼠血清生化、胰岛素抵抗(IR)等指标的变化。结果 1与NAFLD模型组比较,NGP干预组、RSTC干预组用药后血清中总胆固醇(TC)、甘油三酯(TG)、丙氨酸转氨酶(ALT)以及天冬氨酸转氨酶(AST)水平明显降低(P<0.05)。2与NAFLD模型组比较,NGP干预组、RSTC干预组用药后有效改善空腹胰岛素(FINS)水平、明显降低胰岛素抵抗指数(IRI)(P<0.05),2种药物均无明显降糖作用(P>0.05)。3与NAFLD模型组比较,NGP干预组、RSTC干预组用药后肝组织中γ-谷氨酰半胱氨酸甘氨酸(GSH)、超氧化物歧化酶(SOD)水平明显升高、丙二醛(MDA)水平明显下降(P<0.05)。4 NAFLD模型组肝组织内可见大量脂肪变性细胞,细胞核被挤到一侧;NGP干预组、RSTC干预组用药后肝组织内只见少量脂肪变性细胞,胞质内脂滴明显变小,细胞核逐渐移向细胞中央;5NGP干预组与RSTC干预组比较,上述各对应指标之间无统计学差异(P>0.05)。结论三七粉可能通过降脂、改善IR与抗氧化应急等作用途径防治非酒精性脂肪肝病。
[Objective]To investigate the mechanism of notoginseng powder（NGP） in the prevention of non-alcoholic fatty liver disease（NAFLD）.[Methods] Sixty healthy male Kunming mice were randomly and equally divided into four groups： normal control group, NAFLD model group, NGP intervention group, rosuvastatin calcium（RSTC） intervention group. NAFLD mice models were established by feeding high cholesterol diets, NGP was continuously intragastrically administrated to NAFLD model mice, and the changes of indicators such as serum biochemical indicators and insulin resistance（IR） of each group were observed.[Results]1 The leveld of serum total cholesterol（TC）, triglyceride（TG）, alanine aminotransferase（ALT） and aspartate aminotransferase（AST） in NGP intervention group and RSTC intervention group decreased significantly after treatment as compared with NAFLD model group（P〈0.05）. 2After treatment, the fasting insulin（FINS） levels effectively improved and insulin resistance index（IRI） significantly reduced in NGP intervention group and RSTC intervention group as compared with NAFLD model group（P 〈0.05）, but both drugs had no significant hypoglycemic effect（P 〉0.05）. 3 After treatment, the levels ofγ-glutamylcysteinylglycine（GSH） and superoxide dismutase（SOD） significantly increased and malondialdehyde（MDA） level significantly decreased in NGP intervention group and RSTC intervention group as compared with NAFLD model group（ P〈0.05）.4In NAFLD model group, the fatty degeneration of cells was frequently seen in liver tissues, and nuclei was pushed to one side.In NGP intervention and RSTC intervention groups, only a small amount of fatty degeneration cells with significantly smaller nuclei were observed in the liver tissues after treatment, lipid droplets within the cytoplasm significantly diminished, and the nuclei gradually shifted towards the centers of the cells. 5There were no statistically significant differences in the above indicators between NGP intervention group and RSTC intervention group（P〉0.05）.[Conclusion]Notoginseng powder can prevent non-alcoholic fatty liver disease through the pathways such as lipid-lowering and improving insulin resistance and anti-oxidative stress.
出处
《职业与健康》
CAS
2015年第12期1611-1613,共3页
Occupation and Health
基金
2013年十堰市科学技术研究与开发计划项目(项目编号:SYKJ 2013013)
关键词
非酒精性脂肪肝病
三七粉
超氧化物歧化酶
丙二醛
胰岛素抵抗
Non-alcoholic fatty liver disease（NAFLD）
Notoginseng powder（NGP）
Superoxide dismutase（SOD）
Malondialdehyde（MDA）
Insulin resistance（IR）
