摘要
目的:探讨补充维生素C对剧烈运动后人体血清炎症因子水平的影响及ROS/NF-κB信号通路在其中的作用。方法:随机选取20名健康男性在校大学生,分为实验组和对照组。运动开始前分别给予两组大学生维生素C片3.0 g和安慰剂口服。两组大学生分别按照75%VO2max进行跑台运动,持续时间为1 h,并于运动干预开始前10 min、结束后0 h和2 h分别采集静脉血,分离血清和血细胞,并利用ELISA法检测外周血IL-1、IL-6、IL-8含量;由血细胞中分离白细胞后,分别测定白细胞中活性氧(ROS)水平和IKKα激酶活性。结果:剧烈运动后,两组大学生外周血白细胞均明显升高,组间无明显差异;对照组外周血IL-1、IL-6、IL-8含量均表现出逐渐升高的趋势,而实验组未表现出此趋势,其中IL-1水平运动前后无明显变化。此外,两组大学生外周血白细胞中IKKα活性均表现出先升后降的变化趋势,其中实验组升高趋势明显低于对照组。而白细胞中ROS水平却表现出逐渐升高的趋势,其中以对照组升高趋势更明显,组间差异有统计学意义。结论:剧烈运动可以引起白细胞中ROS/NF-κB信号通路活化和血清炎症因子水平升高,而运动前补充维生素C可以有效抑制白细胞中ROS/NF-κB信号通路活化介导的血清炎症因子水平升高。
Objective： To illustrate the effect of Vitamin C on the serum level of inflammatory factors and the role of ROS / NF- κB signaling pathway. Methods： 10 healthy male college students were randomly selected and equally divided into experiment group and control group. Vitamin C（ 3. 0 g）and placebo were given prior to treadmill running exercise for 1 h,the strength of which was equal to75% VO2 max. Venous blood was collected at 10 min before the beginning,0 h and 2 h after the end of the exercise,respectively. The levels of IL- 1,IL- 6 and IL- 8 were detected using ELISA method. The level of reactive oxygen species（ ROS） and the activity of IKKα in white blood cells were also measured. Results： After the strenuous exercise,the leukocyte number in peripheral blood was significantly increased in both groups,however,there was no significant differences. The levels of IL- 1,IL- 6 and IL- 8 in peripheral blood were gradually up- regulated in control group,but not in the experiment group. In addition,the level of IL- 1 was not changed by the exercise. The activity of IKKα elevated soon after the exercise but dropped finally in both groups. The intracellular ROS levels were both gradually raised in the two groups. Similarly,the activity of IKKα and the intracellular ROS levels in the experiment group were both significantly lower compared to the control group. Conclusion： The strenuous exercise could activate ROS / NF- κB signaling pathway and enhance the expression of serum inflammatory factor. Oral intake of Vitamin C prior to the exercise might suppress the expression of serum inflammatory factor induced by ROS / NF- κB signaling pathway.
出处
《山东体育学院学报》
北大核心
2015年第3期62-66,共5页
Journal of Shandong Sport University
基金
陕西省体育局常规课题基金资助项目(编号:13044)
