摘要
以番石榴叶为原料,番石榴叶中番石榴苷、广寄生苷总含量为指标,在单因素试验的基础上,采用响应面法优化番石榴苷、广寄生苷的提取工艺,同时测定不同月份番石榴叶中番石榴苷、广寄生苷总含量,确定最佳采集月份,并研究此月份的提取物对大鼠脂肪细胞的降糖活性及急性毒性。结果表明,最佳提取工艺为时间81min、液料比11∶1、乙醇体积分数64.6%,此条件下番石榴苷、广寄生苷总含量可达3.40(±0.02)mg/g,重复试验表明该最佳工艺条件稳定可行。番石榴叶中番石榴苷、广寄生苷含量随季节变化较大,7月份的含量最高,达到6.74(±0.13)mg/g,因此可选择7月份作为最佳采集月份,且1.6 mg/m L的7月份番石榴叶提取物促脂肪细胞摄取葡萄糖达1.66(±0.23)mmol/L,相比8 U/m L胰岛素具有更好效果。急性毒性试验结果表明,小鼠番石榴叶提取物最大耐受量大于10 g/kg。
Response surface methodology （RSM）was used to optimize the extraction conditions of guaijaverin and avicularin from guava leaves based on slngle-factor tests. The best harvest month was valued according to the content of guaijaverin and avicularin from guava leaves. Besides, the hypoglycemic activity in vitro and the acute toxicity of guava leaves harvested in the best month was evaluated by the isolated rat adipocyte model and mice separately. Results showed that the optimum extraction conditions by RSM were as follows： extraction time of 81 min, ratio of liquid to material of 11 ： 1, ethanol concentration of 64.6%. Under these conditions, the yield of guaijaverin and avicularin was 3.40 （ ± 0.02） mg/g. The repeated tests showed that the optimal conditions were stable and feasible. The total content of guaijaverin and avicularin was different in guava leaves from different months. The highest content of guaijaverin and avicularin was from July, which was up to 6.74 （±0.13）mg/g. 1.6 mg/mL extracts from July promoted adipocyte glucose uptake level to 1.66 （ ± 0.23 ） mmol/L, which was better than 8 U/mL insulin. The maximum tolerated dose of guava leaves extracts in the acute toxicity test was more than 10 g/kg.
出处
《广东农业科学》
CAS
2015年第11期100-107,共8页
Guangdong Agricultural Sciences
基金
广州市科技计划项目(2013J4100075)
国家自然科学青年基金(31201321)
湖南省教育厅项目(13B084)
关键词
番石榴叶
番石榴苷
广寄生苷
响应面分析法
降糖活性
毒性
guava leaves
guaijaverin
avicularin
response surface methodology
hypoglycemic activity
toxicity
