摘要
采用电融合新技术可提高生米卡链霉菌原生质体融合频率,并且在原生质体稳定液中加入2.5mol/L的MgCl_2,比使用PEG助融方法的融合频率高10倍。经过UV灭活高产菌株和另一脯氨酸缺陷型菌株的原生质体的电融合,其融合子的抗生素产量变异幅度大,发酵产物的各组份比例改变,经过选择获得了一新的高产菌株,麦迪霉素产量提高77％,其有效组份A_1比例增加。
The fusion frequency of Streptomyces mycarofaciens protoplast can be increased with electrofusion techniques. When the protoplast stable buffer contained 2.5mol/L MgCl2, the maximum electrofusion frequency(3.4×10-2) was ten times higher than the fusion frequency with polyethyleneglycol-induced(1.9×10-3).By the means of the electrofusion of the protoplasts between UV-inactived high yielding str ain and anauxotrophic strain(Pro-).the antibiotic yield of the fusants was significantly higherand the components of the products were changed as well, more Midecamycin A1 produced than the parent.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1991年第5期323-327,共5页
Chinese Journal of Antibiotics
关键词
生米卡链霉菌
原生质体
电融合
Protoplast electrofusion
Recombination
StrePtomyces mycarofaciens.
