摘要
目的：建立HPLC测定梅花配方颗粒中芦丁、金丝桃苷、异槲皮苷和槲皮素含量的方法。方法：Phenomenex Synergi C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈（A）-0.1%磷酸（B）梯度洗脱,柱温30℃,体积流量1.0 m L·min-1,检测波长360 nm。结果：芦丁、金丝桃苷、异槲皮苷和槲皮素线性范围分别为0.230 2~2.877 5μg（r=0.999 2）,0.134 8~1.685 0μg（r=0.999 5）,0.195 4~2.442 5μg（r=0.999 1）,0.033 8~0.422 5μg（r=0.999 9）线性关系良好,平均加样回收率分别为98.39%,98.71%,100.00%,100.57%;RSD分别为1.7%,1.4%,1.8%,2.0%。结论：该测定方法简便快捷、准确、重复性好,为梅花配方颗粒的质量控制和评价提供了依据。
Objective： To establish an HPLC method for simultaneous determination ofrutin, hyperin, isoquercitrinand quercetinin Mone Flose dispensing granule. Method： HPLC method was performed on a Phenomenex C18 （4.6 mm× 250 ram, 5 p,m） with Acetonitrile （A） -0. 1% phosphoric acid （B） as mobile phase by gradient elution. The flow rate was 1.0 mL ·min, column temperature was 30 ℃ and detection wavelength was 360 nm. Result： Rutin, hyperin, isoquercitrinand quercetin showed good linearity in the ranges of 0.230 2-2.877 5 μg （r=0.9992）, 0.1348-1.6850 μg （r=0.9995）, 0.1954-2.4425 μg （r=0.999 1） and 0.033 8-0. 422 5 μg （r = 0.999 9） with average recoveries of 98.39%, 98.71%, 100.00% and 100.57% , with RSD 1.7% , 1.4% , 1.8% and 2.0% , respectively. Conclusion： The method is rapid, accurate and repeatable, which can provide a reliable way for evaluating the quality of Mone Flose dispensing granule.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第14期49-51,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
佛山市科技创新专项(2013GQ100032)
广东省教育部产学研结合项目(2012B091100183)
关键词
梅花
配方颗粒
芦丁
金丝桃苷
异槲皮苷
槲皮素
Mone Flose
dispensing granule
rutin
hyperin
isoquercitrin
quercetin
