摘要
采用含有4个碳原子臂长的PCB37半抗原衍生物合成PCB37全抗原,以人工抗原免疫BALB/C小鼠,通过鼠-鼠杂交瘤技术制备抗PCB37单克隆抗体。建立间接竞争ELISA（ic-ELISA）分析方法,对抗PCB37单克隆抗体的特性进行评估,最终获得了一株能够稳定分泌抗PCB37单克隆抗体的细胞株。使用该抗体建立了ic-ELISA,在PCB37浓度为0.10~50μg/L时,抑制率与PCB37的浓度呈线性关系,半数抑制浓度（IC50）为1.23μg/L,最低检测限（IC15）为0.027μg/L,与其他物质的交叉反应率〈5.5%。结果表明,成功研制了抗PCB37单克隆抗体,为进一步建立检测PCB37的免疫分析检测方法奠定了基础。
To prepare monoclonal antibody against 3,4,4’-trichlorobiphenyl（ PCB37） in order to develop an immunological test for detecting PCB37 based on monoclonal antibody. Method PCB37 complete antigen was prepared by PCB37 hapten bearing four carbon length carboxylic groups that can be linked to proteins as carrier protein. BABC / c mice were immunized with PCB37 complete antigen. Monoclonal antibodies against PCB37 were prepared by fusion of mouse myeloma sells（ SP2 /0） with spleen cells isolated from immunized BABC / c mouse. An ic-ELISA was established to evaluate the characterization of this monoclonal antibody. A hybridoma cell line producing antibody against PCB37 was prepared. Based on this monoclonal antibody,an ic-ELISA for detecting PCB37 was developed. Good linearity was achieved within a range of 0. 1 ~ 50 μg / L. The half-maximal inhibition concentration（ IC50） was 1. 23 μg/L,and the limit of detection（ IC15） was 0. 027 μg/L. The cross-reactivity with other congeners was within 5. 5%. A monoclonal antibody against PCB37 was prepared,which provided a foundation for further development of immunoassay to detect PCB37 in the environment.
出处
《化学试剂》
CAS
北大核心
2015年第7期643-646,共4页
Chemical Reagents
