摘要
目的:采用高效液相色谱分析法测定不同灌药周期兔药物血清中人参的有效成分人参皂苷Rg1血药浓度,确立中药药物血清制作中的灌药周期。方法:色谱柱为Cosmosil C18柱(4.6 mm×250 mm,5μm),人参皂苷Rg1流动相为甲醇∶0.1%磷酸(60∶40),检测波长203 nm。结果:随着灌药时间的延长,血清中的人参皂苷Rg1的血药浓度逐渐增加,在灌胃3 d血药浓度最低,灌胃7 d、14 d、21 d血药浓度均明显高于3 d(P<0.01),21 d与14 d相比差异无统计学意义(P>0.05)。结论:高效液相色谱法简便、准确,重复性好,为中药药物血清实验中实验动物的灌药周期提供实验依据,并能够有效控制四君子汤拆方人参的质量。
Objective：To detect the blood drug concentration of ginsenosides Rg1 in different feeding cycles by high performance liquid chromatography（ HPLC） for determining the feeding cycle in the production of rabbit ginseng serum. Methods：The C18 column （4. 6 mm ×150 mm,5 μm） was used,the Methanol-0. 1% phosphate（60：40） was the mobile phase of ginsenosides Rg1,and the detection wave-length was 203 nm. Results：With the prolonging of feeding cycle, the blood drug concentration of ginsenosides Rg1 increased proportionally,the concentrations of 7 days,14 days and 21 days were significantly higher than the concentrations of 3 days （P〈0.01）,the difference between 21 days and 14 days was not statistically significant（P 〉0. 05）. Conclusions：The HPLC is simple,accurate and reproducible, which can provide the experimental evidence in determining the feeding cycle of serum test, and effectively control the quality of ginseng.
出处
《蚌埠医学院学报》
CAS
2015年第6期701-703,707,共4页
Journal of Bengbu Medical College
基金
国家自然科学基金项目(8117386)
蚌埠医学院研究生科研创新计划项目(Byycxz1314)
关键词
药物/分析
人参皂苷RG1
高效液相色谱法
drug/analysis
ginsenosides Rgl
high performance liquid chromatography
