摘要
目的：探讨社区获得性肺炎（CAP）患者血清和支气管肺泡灌洗液（BALF）中炎症因子白细胞介素（IL）-6、IL-8和IL-10水平的变化及其临床意义。方法：选取CAP患者50例（CAP组），入院第1天进行临床肺部感染评分，〈6分者为轻症组，1〉6分者为重症组；选取同期10名健康志愿者为对照组。双抗体夹心酶联免疫吸附法测定CAP组入院第1天以及对照组血清和BALF中IL-6、IL-8、IL-10水平，同法测定CAP组入院第7、30天血清中IL-6、IL-8和IL-10水平。结果：CAP组入院第1天血清和BALF中IL-6、IL-8、IL-10水平均高于对照组（P〈0．05～P〈0．01）。CAP重症组血清中IL-6、IL-10和BALF中IL-6、IL．8、IL-10浓度均明显高于轻症组（P〈0．01）。CAP组血清IL-6和IL-10水平均随病程呈下降趋势（P〈0．01），在人院第7天和第30天均达到对照组水平（P〉0．05）；而CAP组血清中IL-8水平在入院第30天仍保持较高水平，显著高于对照组（P〈0．01）。结论：IL-6、IL-8和IL-10均参与了CAP患者局部和系统的炎症反应，BALF中IL-6、IL-8、IL-10和血清中IL-6、IL-10的水平可反映肺部感染的严重程度，检测血清中IL-6、IL-8和IL-10对CAP早期诊断有一定的临床价值。
Objective ：To explore the levels of IL-6, IL-8 and IL-10 in serum and bronchoalveolar lavage fluid（BALF） of patients with community-acquired pneumonia（CAP）, and its clinical significance. Methods： The clinical pulmonary infection score of 50 patients with CAP were detected on the first day after admission. The patients with CPIS less than 6 and equal to or more than 6 were divided into the light group and severe group,respectively. Ten healthy people were set as the control group. The levels of IL-6 ,IL-8 and IL-10 in serum and BALF of the patients with CAP were determined by radioimmunoassay on the first day after admission. The levels of IL-6, IL-8 and IL-10 in serum of the patients with CAP were also measured on day 7 and 30 of admission. Results：The levels of IL-6,IL-8 and IL-10 in serum and BALF of CAP patients were significantly higher than those in control group on the first day after admission（ P 〈 0.05 to P 〈 0. 01 ）. The levels of IL-6 and IL-10 in serum and levels of IL-6, IL-8, IL-10 in BALF in severe CAP group were significantly higher in light CAP patients（ P 〈 0.01 ）. With the disease course prolonging, the levels of IL-6 and IL-10 decreased（ P 〈 0.01） ,which arrived at the level of the control group on day 7 and 30 of admission（P 〉0.05）. The level of IL-8 in serum of CAP patients remained at a high level on day 30 of admission, which was significantly higher than that in control group （ P 〈 0. 01 ）. Conclusions ：The IL-6, IL-8 and IL-10 were involved in the pathogenesis of community-acquired pneumonia. The levels of IL-6 and IL- 10 in serum and levels of IL-6, IL-8 and IL-10 in BALF can reflect the severity of pulmonary infection. The detection of IL-6, IL-8 and IL-10 in serum has certain clinical value in the early diagnosis of CAP.
出处
《蚌埠医学院学报》
CAS
2015年第6期740-743,共4页
Journal of Bengbu Medical College
关键词
肺炎
肺泡灌洗液
白细胞介素
pneumonia
bronchoalveolar lavage fluid
interleukin
