摘要
CD4+T淋巴细胞是人体免疫缺陷病毒(HIV)的主要感染细胞,慢性HIV感染者逐渐耗尽CD4+T淋巴细胞,使免疫系统变弱,导致获得性免疫缺陷综合症(AIDS),因此,CD4+T淋巴细胞的数量对HIV/AIDS的诊断和治疗至关重要。全球范围内HIV/AIDS正处于快速增长期,现有的CD4+T淋巴细胞计数检测方法由于仪器昂贵、操作复杂、检测成本高,不利于疾病诊疗的普及与推广。为实现低成本、方便、快捷的临床检测,基于微流控芯片的CD4+T淋巴细胞计数检测方法与技术的研究正日益受到人们的重视。本文在回顾传统CD4+T淋巴细胞检测方法的基础上,综述、归纳了基于微流控芯片的CD4+T淋巴细胞计数方法,在全面分析其技术特点的基础上,进一步评述了其综合性能、适用范围、及典型优缺点。最后,本文针对基于微流控芯片的CD4+T淋巴细胞计数检测技术的发展趋势及商业化应用前景进行了讨论和展望。
CD4＋ T lymphocytes are the main infected targets of the Human Immunodeficiency Virus（ HIV） in bodies. CD4 level w ill gradually decrease in HIV hosts w hen their immune systems are becoming more and more w eak,w hich finally causes Acquired Immune Deficiency Syndrome（ AIDS）. Therefore,the number of CD4＋ T lymphocytes for HIV hosts at different stages is critical to the diagnosis and therapy of HIV/AIDS. For example,Antiretroviral therapy（ ART） normally requires four periodical CD4 ＋ T tests in one year for clinical diagnosis and treatment. Population w ith HIV/AIDS is increasing significantly all over the w orld in the past ten years. The existing methods for CD4＋ T lymphocyte counting are unavailable to developing countries or undeveloped areas because of expensive devices, complicated procedures and high cost. To solve this problem, based on microfluidic technology,new CD4＋ T lymphocyte counting methods are being intensively studied for low cost,rapid and convenient CD4＋ T lymphocyte detection in the point-of-care test（ POCT）. After a brief introductionon traditional methods,this paper review s and summarizes CD4 ＋ T lymphocyte counting methods based on microfluidic chips. The typical technical characteristics of chip-based CD4＋ T lymphocyte counting methods are identified and grouped,and furthermore,their general performance,application area,and major advantages w ith disadvantages are discussed and evaluated in details. Finally, an outlook and conclusion for research,development and commercialization of CD4＋ T lymphocyte counting based on microfluidic chip are given after a detailed discussion.
出处
《化学进展》
SCIE
CAS
CSCD
北大核心
2015年第7期870-881,共12页
Progress in Chemistry
基金
国家自然科学基金项目(No.81371711)
中央高校基本科研业务费专项资金(No.ZZ1329
YS1404)~~
