摘要
本实验建立了颐脑解郁颗粒中栀子苷和五味子醇甲的高效液相色谱测定方法。实验中采用Eclipse XDB-C18色谱柱（250 mm×4.6 mm，5 μm），流动相甲醇-水，梯度洗脱，体积流量1.0 mL/min，柱温25 ℃，检测波长0-20 min为238 nm，20-40 min为250 nm。结果表明栀子苷在0.2864-2.8640 μg范围内线性关系良好，r=0.9997，平均回收率为98.82%，RSD为1.53%；五味子醇甲在0.1062-1.0620 μg范围内线性关系良好，r = 0.9999，平均回收率为 99.23%，RSD为2.29%；3批样品中栀子苷的平均含量为8.53 mg/g，五味子醇甲的平均含量为2.82 mg/g。该方法简便准确，专属性强，重复性好，可有效控制颐脑解郁颗粒的质量。
This experiment was aimed to establish a RP-HPLC method for determining the contents of jasminoidin and schisandrae in Yinao Jieyu Granules.The experiment was carried out on an Eclipse XDB-C18 column （250 mm×4.6 mm,5 μm） with gradient elution using methanol-water as mobile phases.The flow rate was 1.0 mL/min and the column oven temperature was set at 25 ℃.The detection wavelength was 238 nm （0-20 min） and 250 nm （20-40 min）.The experimental result indicated that the linear range of jasminoidin was 0.2864-2.8640 μg （r = 0.9997）,the average recovery was 98.82% and RSD was 1.53% （n=6）;The linear range of schisandrae was 0.1062-1.0620 μg （r = 0.9999）,the average recovery was 99.23% and RSD was 2.29% （n=6）.The average contents of jasminoidin and schisandrae in 3 batches of samples were 8.53 mg/g and 2.82mg/g,respectively.The developed RP-HPLC method was simple,accurate,specific and reproducible. It can be used to control the quality of Yinao Jieyu Granules effectively.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第7期1215-1218,共4页
Natural Product Research and Development
基金
国家"重大新药创制"科技重大专项(2014ZX09301307-012)
