摘要
目的探讨血小板二磷酸腺苷(adenosine diphosphate,ADP)受体亚基12(P2Y12)基因单核苷酸多态性位点rs2046934、rs6787801、rs6798347及rs6801273与冠状动脉粥样硬化性心脏病(冠心病)发生的相关性。方法入选455例冠心病患者和90例健康对照。所有冠心病患者均行冠状动脉造影检查或冠状动脉计算机断层扫描血管造影确诊冠心病。应用Mass ARRAY时间飞行质谱技术对P2Y12基因的单核苷酸多态性位点进行基因分型。比较两组在基因型分布及等位基因频率上的差别。结果冠心病组与对照组在rs2046934位点的基因型分布(CC:2.4%vs.4.4%、TT:71.0%vs.74.4%、CT:26.6%vs.21.2%)和等位基因频率(C等位基因:15.7%vs.15.0%、T等位基因:84.3%vs.85.0%)比较,差异无统计学意义(P>0.05)。两组rs6787801位点的基因型分布(GG:17.4%vs.17.8%、AA:36.9%vs.31.3%、GA:45.7%vs.51.1%)和等位基因频率(G等位基因:40.2%vs.43.3%、A等位基因:59.8%vs.56.7%)比较,差异无统计学意义(P>0.05)。两组rs6798347位点的基因型分布(AA:9.1%vs.6.7%、GG:41.6%vs.52.2%、AG:49.3%vs.41.1%)和等位基因频率(A等位基因:29.9%vs.27.2%、G等位基因:70.1%vs.72.8%)比较,差异无统计学意义(P>0.05)。两组rs6801273位点的基因型分布(CC:13.4%vs.10.0%、TT:42.6%vs.50.0%、CT:44.0%vs.40.0%)和等位基因频率(C等位基因:35.4%vs.30.0%、T等位基因:64.6%vs.70.0%)比较,差异无统计学意义(P>0.05)。结论 P2Y12基因单核苷酸多态性位点(rs2046934、rs6787801、rs6798347及rs6801273)与冠心病发生无相关性。
Objectives To investigate the correlation between platelet adenosine diphosphate acceptor subunit 12（P2Y12） gene polymorphisms（rs2046934, rs6787801, rs6798347 and rs6801273） and coronary heart disease.Methods Totally 455 patients with coronary artery disease who were diagnosed by coronary angiography or coronary artery computed tomography angiography and 90 healthy control individuals were enrolled in this study. The single nucleotide polymorphisms（rs2046934, rs6787801, rs6798347 and rs6801273） of P2Y12 gene were detected by Mass ARRAY Time of Flight Mass Spectrometry. The genotypes and allele frequencies between the two groups were compared. Results Genotypes and frequencies of P2Y12 gene polymorphisms rs2046934 in coronary heart disease group and control group were as below ： CC genotype 2.4% vs. 4.4%, TT genotype 71.0% vs. 74.4%, CT genotype26.6% vs. 21.2%; and C allele frequencies were 15.7% vs. 15.0%, T allele frequencies were 84.3% vs. 85.0%.There were no significant differences between the two groups（P〉0.05）. As for gene polymorphisms rs6787801, GG genotype was 17.4% vs. 17.8%, AA genotype 36.9% vs. 31.3%, GA genotype 45.7% vs. 51.1%; and G allele frequencies were 40.2% vs. 43.3%, A allele frequencies were 59.8% vs. 56.7%. There were no significant differences in genotypes and frequencies of P2Y12 gene polymorphisms rs6787801 between these two groups（P〉0.05）. As for gene polymorphisms rs6798347, AA genotype was 9.1% vs. 6.7%, GG genotype 41.6% vs. 52.2%, AG genotype 49.3%vs. 41.1%; and A allele frequencies were 29.9% vs. 27.2%, G allele frequencies were 70.1% vs. 72.8%. There were no significant differences in genotypes and frequencies of P2Y12 gene polymorphisms rs6798347 between these two groups（P〉0.05）. As for gene polymorphisms rs6801273, CC genotype was 13.4% vs. 10.0%, TT genotype 42.6% vs.50.0%, CT genotype 44.0% vs. 40.0%; and C allele frequencies were 35.4% vs. 30.0%, T allele frequencies were64.6% vs. 70.0%. There were no significant differences in genotypes and frequencies of P2Y12 gene polymorphisms rs6801273 between these two groups（P〈0.05）. Conclusions Single nucleotide polymorphisms of P2Y12 gene in rs2046934, rs6787801, rs6798347 and rs6801273 are not associated with coronary heart disease.
出处
《岭南心血管病杂志》
2015年第2期163-166,248,共5页
South China Journal of Cardiovascular Diseases
关键词
冠状动脉疾病
血小板二磷酸腺苷受体亚基12
多态性
单核苷酸
coronary heart disease
platelet adenosine diphosphate acceptor subunit 12
polymorphism
single nucleotide
