摘要
以蛹虫草中类胡萝卜素的提取得率为考察指标,分别运用酸热提取法、超声提取法、酸热超声提取法、微波提取法、酸热微波提取法、丙酮提取法提取类胡萝卜素,比较6种方法的提取得率,得出酸热超声提取法是较好的提取方法。采用酸热超声提取法,设盐酸浓度、酸浸时间、沸水浴时间、超声功率、超声温度和超声时间等6个单因素试验,结合L27(313)正交试验设计,优化提取工艺参数。得出酸热超声提取法最优提取条件为盐酸浓度2.25 mol/L,酸浸时间30 min,沸水浴时间6 min,超声功率400 W,超声温度25℃,超声时间25 min,类胡萝卜素提取得率可达1 134.88μg/g;影响提取得率大小的因素依次为超声功率、酸浸时间、盐酸浓度、沸水浴时间、超声温度、超声时间。
By taken extraction efficiency ofcarotenoids from Cordyceps militaris as an index, six different extraction approaches were compared for selecting the best one, they were heat acid extraction technique, ultrasonic wave extraction technique, heat acid-ultrasonic wave extraction technique, microwave extraction technique, heat acid microwave extraction technique and acetone extraction technique, The results showed that heat acid-ultrasonic wave extraction technique was the better one for extract carotenoids in C.militaris. The optimum approach was adopted to conduct six single-factor experiments, including HC1 concentration, time of soaking in HCl, heating time, ultrasonic power, ultrasonic extraction temperature and ultrasonic extraction time, then the L_27（3^13） orthogonal test was designed to optimize the extraction conditions. Results indicated that the extraction efficiency could reach up to 1 134.88 μg/g under these conditions, they were the 2.25 mol/L concentration of HCl, 30 min of soaking time in HCl, 400 W of ultrasonic power, 6 min heating time, 25 ℃ ultrasonic extraction temperature and 25 rain ultrasonic extraction time; These conditions ranked as follows in turn： ultrasonic power, time of soaking in HCl, HCl concentration, heating time, ultrasonic extraction temperature, ultrasonic extraction time.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第4期416-422,共7页
Journal of Hunan Agricultural University(Natural Sciences)
基金
江苏省农业科技自主创新资金项目(CX(14)2137)
关键词
蛹虫草
类胡萝卜素
酸热超声提取法
工艺优化
Cordyceps militaris
carotenoids
heat acid-ultrasonic wave extraction technique
process optimization
