摘要
[目的]建立一种壳聚糖去氧胆酸聚合物(deoxycholic acid grafted chitosan,CS-DCA)接枝率测定的新方法。[方法]分别建立并采用线性电位滴定法(linear potentiometric titration,LPT)和2,4,6-三硝基苯磺酸(2,4,6-trinitrobenzenesulfonicacid,TNBS)法测定CS-DCA的接枝率,以氢核磁共振波谱(1H-nuclear magnetic resonance,1H-NMR)法为参比,比较分析可替代的接枝率测定方法。[结果]比较CS-DCA的接枝率测定数据,经典方法1H-NMR法与LPT无统计学差异(P>0.05),而与TNBS法具有显著性差异(P<0.05),表明LPT与经典方法的准确度相当;LPT的最大相对误差为3.8%(n=15),而TNBS法最大相对误差为12.8%(n=15),提示LPT有更高的精密度。[结论]LPT可作为一种新的经济、快速且准确的CS-DCA接枝率测定方法。
Objective]To establish a new method for grafting ratio（GR） analysis of deoxycholic acid grafted chitosan（CS-DCA）. [Methods]In this study, linear potentiometric titration（LPT） and 2,4,6-trinitrobenzenesulfonicacid（TNBS） were introduced to measure the GR of CS-DCA, using the 1H-nuclear magnetic resonance（1H-NMR） as reference, the results obtained by the two methods were analyzed to find a method to substitute H-NMR. [Results]There was no significant difference of the data determined by 1H-NMR and LPT（P〉0.05）, however, it didn＇t apply to 1H-NMR and TNBS（P〈0.05）, the results indicated that LPT had equivalent accuracy of 1H-NMR. Using 1H-NMR method as the standard reference, the relative error was no more than 3.8% for the method of LPT, and the maximum relative error for the method of TNBS was up to 12.8%, it meant that LPT had higher precision. [Conclusion]LPT is an economical, rapid and accurate method for the GR analysis of CS-DCA.
出处
《浙江中医药大学学报》
CAS
2015年第5期372-377,共6页
Journal of Zhejiang Chinese Medical University
基金
浙江省新世纪151人才工程(第二层次)~~
关键词
接枝率测定方法
壳聚糖去氧胆酸聚合物
线性电位滴定法
准确度
精密度
method for grafting ratio analysis
deoxycholic acid grafted chitosan
linear potentiometric titration
accuracy
precision
