摘要
建立了同时测定食品中抗氧化剂TBHQ、BHA和BHT的高效液相色谱法。样品经甲醇提取后,以C18烷基柱（150 mm×4.6 mm,粒径5.0μm）为分析柱,甲醇-水（体积比85∶15）为流动相进行洗脱,SPD-20A紫外检测器检测,波长280 nm。结果表明,TBHQ、BHA和BHT在2~26μg/mL范围内有良好的线性关系,线性相关系数为0.9992~0.999 5,方法检出限分别为2.27×10-3,1.95×10-3和4.82×10-3μg/mL,加标回收率为86.4%~108.6%,相对标准偏差（RSD）小于7.23%（n=6）。
Build up simultaneous determination of butylated hydroxytoluene （BHT）, butyl hydroxy anisd （BHA） and tert-butylhydroquinone （TBHQ） in food by high performance liquid chromatography. The sample was extracted by methyl alcohol, C18 column （150 mm×4.6 mm, particle size of 5.0 μm） as analytical column, methanol water （volume ratio was 85 ： 15） was used as mobile phase in gradient elution, SPD-20A ultraviolet detector, the wavelength of 280 nm. The results showed that TBHQ, BHA and BHT had good linear relationship in the range of 2-26 μg/mL; the linear correlation coefficient was 0.999 2-0.999 5; the detection limits were 2.27× 10-3, 1.95× 10-3 and 4.82× 10-3 μg/mL; the recovery was 86.4%-108.6%; the relative standard deviation （RSD） was less than 7.23% （n=6）.
出处
《食品工业》
北大核心
2015年第7期273-275,共3页
The Food Industry
基金
国家级大学生创新创业训练项目(编号20141012499)
山西省大学生创新创业训练项目(编号2014382)
