摘要
目的探讨髓鞘碱性蛋白(myelin basic protein,MBP)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)在实验性胰性脑病大鼠模型血清及脑脊液中水平的变化,分析各因子与胰性脑病发生发展的关系,为临床胰性脑病的诊治提供实验依据。方法选取40只SD大鼠随机分为假手术组(SO组,n=10)和胰性脑病组(PE组,n=30),经十二指肠乳头逆行胰胆管穿刺注射生理盐水(SO组)或5%牛磺胆酸钠(PE组)诱导建立大鼠胰性脑病模型。SO组大鼠于手术后1 d全部处死,PE组分别于造模后1 d、3 d及7 d各处死10只,取各组大鼠的脑组织和胰腺组织,观察其病理学改变,并观察脑微血管内白细胞聚集及附壁现象;检测脑组织含水量,以及血清和脑脊液中MBP、TNF-α和TL-6的含量。结果 PE组大鼠造模后随时间延长,脑神经细胞水肿及神经纤维脱髓鞘改变明显;血清及脑脊液中MBP、TNF-α和IL-6的含量在造模后1 d、3 d及7 d均明显高于SO组(P<0.05),且随时间的延长逐渐升高,但经两两比较,显示上述3项指标的变化趋势有所不同。结论 MBP、TNF-α和IL-6对胰性脑病脑损害的发生和发展发挥了协同作用;通过检测血液及脑脊液中MBP、TNF-α及IL-6的含量可对胰性脑病病情进行诊断和评估。
Objective To discuss the changes of myelin basic protein （MBP）, tumor necrosis factor-a （TNF-α）, and interleukin-6 （IL-6） in serum and cerebrospinal fluid of experimental pancreatic encephalopathy rat model, analyze the relationship between each factor and the occurrence and development of pancreatic encephalopathy, and to provide the experimental basis for clinical diagnosis and treatment of pancreatic encephalopathy. Methods Selecting 40 SD rats were randomly divided into sham operation group （SO group, n=10） and pancreatic encephalopathy group （PE group, n=30）, respectively by the duodenal papilla retrograde pancreatic puncture injection of saline solution or 5% sodium taurocholic acid induced rat pancreatic encephalopathy model were set up. The rats in SO group were sacrificed on 1 d, and the PE group were sacrificed ten rats on 1 d, 3 d, and 7 d, respectively after surgery. The brain and pancreatic tissues of rats in each group were taken to observe the pathological changes of the rats and the brain white blood cells within microvessels gathered and coanda phenomenon. The water content of brain tissues, and the contents of MBP, TNF-α and TL-6 in serum and cerebrospinal fluid were detected. Results The changes of brain nerve cell edema and nerve fiber demyelination were obvious in PE group rats after surgery with the extension of time. The contents of MBP, TNF-αand TL-6 in serum and cerebrospinal fluid on 1 d, 3 d, and 7 d after surgery in PE group were significantly higher than that SO group （P〈0.05）, and gradually increased with the extension of time. But by two two compared, the change trend of theabove three indicators were different. Conclusions MBP, TNF, and IL-6 on the occurrence and development of brain damage of pancreatic encephalopathy play a synergistic effect. To detecte the MBP, TNF-α, and IL-6 content in blood and cerebrospinal fluid could be diagnosed and evaluated the pancreatic encephalopathy.
出处
《中国普外基础与临床杂志》
CAS
2015年第7期816-821,共6页
Chinese Journal of Bases and Clinics In General Surgery
基金
内蒙古自治区高等学校科学研究项目(项目编号:NJ10141)~~
