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miR-130b促进人胃癌BGC823细胞增殖的研究 被引量：2

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摘要 目的观察靶向干扰miR-130ba对人胃癌细胞系BGC823的增殖作用及相关机制。方法培养人胃癌细胞系BGC823,分成正常组、对照组、抑制组,采用脂质体2000分别转染PBS、miR-130b错义链(50n M)、miR-130b抑制剂(50n M),使用real time PCR方法测定转染后miR-130b水平,CCK8法检测转染后胃癌细胞增殖,Western blot检测转染后增殖细胞核抗原蛋白水平。结果转染48 h后,抑制组胃癌细胞的miR-130b mRNA明显低于正常组、对照组(P<0.01),同时抑制组胃癌细胞CCK8 OD及增殖细胞核抗原蛋白水平均低于正常组、对照组(P<0.05)。结论 miRNA-130b可以促进胃癌细胞BGC823的增殖和增殖细胞核抗原蛋白表达。
作者 陈华 胡海军 单爱军
机构地区 暨南大学第二临床医学院 深圳市人民医院急诊科 深圳市人民医院胃肠外科
出处 《齐齐哈尔医学院学报》 2015年第17期2506-2507,共2页 Journal of Qiqihar Medical University
关键词 胃癌细胞 miRNA-130b 细胞增殖
分类号 R735.2 [医药卫生—肿瘤]
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参考文献10

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齐齐哈尔医学院学报
2015年 第17期

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