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整合素αvβ_3在IGF-II诱导的宫颈癌细胞株增殖中的作用

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摘要 目的探讨整合素αvβ3在IGF-II诱导的宫颈癌细胞株增殖中的作用。方法用MTT法观察细胞增殖,用Western blot测定细胞ERK1/2(p42/44MAPK)磷酸化表达。结果用IGF-IR单克隆抗体(1,5,10μmol/L)和αvβ3单克隆抗体(10,15,20μmol/L)预处理30分钟可显著抑制IGF-II(20 ng/ml)诱导的SiHa细胞增殖,并呈剂量依赖性。IGF-II(100 ng/ml)可诱导SiHa细胞p42/44MAPK磷酸化表达,IGF-IR(10μmol/L)及αvβ3(15μmol/L)单克隆抗体可显著抑制IGF-II诱导的Siha细胞p42/44MAPK磷酸化。结论 IGF-II可激活宫颈癌SiHa细胞株p42/44MAPK信号通路,整合素αvβ3与IGF-IR信号通路上存在着交互作用。
作者 王瑶 金鑫
机构地区 黑龙江省大庆油田总医院
出处 《齐齐哈尔医学院学报》 2015年第18期2658-2660,共3页 Journal of Qiqihar Medical University
关键词 宫颈癌 IGF-IR信号通路 整合素ΑVΒ3
分类号 R392.1 [医药卫生—免疫学]
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参考文献8

  • 1Schoell WM, Janicek MF, Mirhashemi R. Epidemiology and biology of cervical cancer[ J]. Semin. Surg. Oncol, 1999,16:203- 211.
  • 2Ferenczy A, Franeo E. Persistent human papillomavirus infection and cervical neoolasia[ J]. Lancet Oncol,2002,3 : 11-16.
  • 3田芳,章雄文,林莉萍,丁健.整合素与生长因子受体之间的交互作用[J].中国癌症杂志,2005,15(4):392-394. 被引量：3
  • 4Pavelic K, Bukocic D, Pavelic J. The role of insulin-ike growth factor 2 and its receptors in human tumors [ J ]. Mol Med,2002,8 (12) :771-780.
  • 5Grinberg A, Cohen P. Role of insulin-like grow factors and their binding proteins in growth control and carcinogenesis [ J ]. J CellInst, 2003,95 ( 4 ) : 291-302.
  • 6Moorehead RA, Sanchez OH, Baldwin RM, et al. Transgenic overexpression of IGF-II induces spontaneous lung tumors a model for human lung adenocarcinoma [ J ]. Oncogene, 2003,22 ( 6 ) : 853- 857.
  • 7Shen MR, Hsu KF, Chen YF, et al. Insulin-like growth factor 1 is a potent stimulator of cervical cancer cell invasiveness and proliferation which is modulated by otv[3s integrin signaling [ J ]. Carcinogenesis ,2006.
  • 8Swindle CS, Tran KT, Johnson, et al. Epidermal growth factor (EGF)-like repeats of human tenasein-C as ligands for EGF receptor[ J]. Cell Biol, 2001,154:459-468.

二级参考文献18

  • 1Miranti CK, Brugge JS. Sensing the environment: a historical perspective on integrin signal transduction[J]. Nat Cell Biol,2002,4(4) :E83-E90.
  • 2Vinogradova O Vaynberg J, Kong X, et al. Membrane-mediated structural transitions at the cytoplasmic face during integrin activation [J]. Proc Natl Acad Sci U S A,2004,101 (12) :4094-4099.
  • 3Damsky CH, Ilic D. Integrin signaling: it's where the action is[ J]. Curr Opin Cell Biol,2002,14(5 ) :594-602.
  • 4Comoglio PM, Boccaccio C, Trusolino L. Interactions between growth factor receptors and adhesion molecules: breaking the rules [ J ]. Curr Opin Cell Biol,2003,15 (5) :565-571.
  • 5Leitinger B, Hogg N. The involvement of lipid rafts in the regulation of integrin function [ J ]. J Cell Sci, 2002,115 ( Pt 5 ) : 963-972.
  • 6Decker L, Baron W, Ffrench-Constant C. Lipid rafts: microenvironments for integrin-growth factor interactions in neural development [ J ]. Biochem Soc Trans,2004,32 ( Pt3 ) :426430.
  • 7Schneller M, Vuori K, Ruoslahti E. Alphavbeta3 integrin associates with activated insulin and PDGFbeta receptors and potentiates the biological activity of PDGF [ J ]. EMBO J, 1997,16(18) :5600-5607.
  • 8Giancotti FG, Tarone G. Positional control of cell fate through joint integrin/receptor protein kinase signaling [ J ]. Annu Rev Cell Dev Biol,2003 ,19 : 173-206.
  • 9Lee JW, Juliano R. Mitogenic signal transduction by integrinand growth factor receptor-mediated pathways [ J ]. Mol Cells,2004,17 (2) :188-202.
  • 10Oshima K, Ruhul Amin AR, Suzuki A, et al. SHPS-1, a multifunctional transmembrane glycoprotein [ J]. FEBS Lett ,2002,519(1-3) :1-7.

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齐齐哈尔医学院学报
2015年 第18期

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