摘要
目的:了解肺泡形成基因P311在支气管肺发育不良(BPD)中的作用;方法:64只早产昆明小鼠随机均分为空气组和高氧组,空气组置于室内,高氧组置于密闭的氧浓度>90%的氧舱中复制BPD动物模型;分别于造模后第1、3、7及14天取小鼠肺组织,分别采用苏木精-伊红染色观察小鼠肺组织病理改变鉴定造模是否成功,实时荧光定量聚合酶链式反应(Real-time PCR)法检测P311基因mRNA表达水平。结果:早产小鼠肺组织发育不成熟,高氧组随着吸氧时间的延长,小鼠表现反应迟钝、体重增长缓慢、毛发发涩无光泽、鼻尖发绀、呼吸急促,肉眼见肺组织表面有散在甚至弥漫的淤血点、小血管扩张充血,第3天镜下见肺泡腔内有炎性细胞浸润,第7天肺泡腔增大、肺间质增生,第14天肺泡腔内间质增生、少数肺泡融合、肺泡数量减少、肺泡结构简单化,说明BPD模型造模成功;第3天时2组P311基因mRNA表达量最高,随后下降;高氧组P311基因mRNA表达水平均高于同一时点空气组(P<0.05)。结论:长时间吸入高浓度氧成功构建支气管肺发育不良的动物模型,P311基因可能是影响肺损伤修复的调控因子。
Objective： To investigate the function of alveoli formation gene P311 in bronehopulmo- nary dysplasia （BPD）. Method： A total of 64 premature mice were divided into air group and high oxygen group; air group was placed in the normal air, high oxygen group was placed in airtight oxygen chamber with oxygen concentration 90% to make BPD model. All subjects were taken the lung tissue 1,3,7 and 14 days after molding. HE staining was used to observe the lung tissue pathological changes and authenticate whether the model building was successful. The P311 gene expression level was detect by real - time quantitative polymerase chain reaction. Results： The lung tissue of premature mice did not develop mature; with the extension of time that the high oxygen group mice showed following reac- tions ： slow reaction, slow growth in weight, lackluster hair, cyanotic nasal tip, short breath. The lung tissue surface had congestion points scattered, dilation and congestion of small blood vessels could be observed by naked eyes. 3 days after molding, the alveolar cavity was infiltrated with inflammatory cells, 7 days after molding, alveolar space increased and lung interstitial hyperplasia were found. Alveolar interstitial hyperplasia, some alveolar fusion and alveolar structure simplified 14 days after molding. These explained the model building was successful. The P311 gene levels in high oxygen group and air group increased on the 3rd day and then decreased. Compared with the air group, the P311 gene expression level was higher in high oxygen group （ P 〈 0.05 ）. Conclusion： Inhaled high concentration oxygen for a long time can successfully build BPD model. P311 gene may be a regulatory factor of lung injury repair.
出处
《贵阳医学院学报》
CAS
2015年第7期688-691,共4页
Journal of Guiyang Medical College
基金
贵州省科技厅科研项目[黔科合J字(2011)2250号]
关键词
肺泡
支气管肺发育不良
肺泡形成基因
动物模型
基因表达
alveoli
bronchopulmonary dysplasia
alveoli formation gene
animal model
gene ex-pression
