摘要
为稳定并提高萨福克羔羊卵泡发育效率,通过肌肉注射FSH和PMSG的方法,对可能影响萨福克羔羊激素诱导效果如羔羊日龄、体重、健康状况和季节等因素进行研究。结果显示：1）5-6周龄萨福克羔羊经激素处理后的平均卵泡数（114.18vs 22.17）、回收的卵母细胞数（82.54vs 12.83）以及可用卵母细胞数（72.79vs 10.83）极显著高于8-9周龄组（P〈0.01）;2）体重在8-18kg之间的5-6周龄羔羊,不影响处理效果（P〉0.05）;3）有疾病史的羔羊处理效果显著低于健康羔羊组（P〈0.01）;4）春、秋季节对羔羊卵泡发育及回收的卵母细胞数没有影响（P〉0.05）,但春季羔羊卵母细胞体外受精卵裂率（54.31%vs 73.93%）和囊胚率（21.34%vs 35.73%）显著低于秋季（P〈0.05）;5）秋季羔羊胚胎移植受胎率（51.33%vs 26.07%）显著高于春季（P〈0.05）;6）移植早期胚胎或囊胚期胚胎对受胎率没有影响（P〉0.05）。选择5-6周龄、体重在8-18kg之间、无疾病史的萨福克羔羊,能显著提高卵泡发育效率;秋季羔羊卵母细胞发育能力显著高于春季;相同季节内,移植早期胚胎或囊胚期胚胎受胎率间没有差异。
In order to stabilize and improve the lamb oocytes in vitro production efficiency,the influence of lamb age,weight,health status,season and other factors on Suffolk lamb hormone was studied.The results showed that：1）The average number of follicles of the 5-6weeks aged Suffolk lambs was significantly higher than those of 8-9weeks aged group（P〈0.01）after treated by hormone（114.18 vs 22.17）、oocytes recovered（82.54 vs 12.83）and usable oocytes（72.79 vs 10.83）;2）There is no significant effect for those lambs weighed between 8-18kg（P 〉0.05）;3）The effects were significantly lower in those lambs with a history of disease than those healthy lambs（P〈0.01）;4）There is no significant season effect（Spring vs.autumn）on lamb follicular development and oocyte recovery（P〉0.05）.However,the cleavage rate（54.31% vs 73.93%）and blastocyst rate（21.34% vs 35.73%）in the spring was significantly lower than in autumn（P〈0.05）;5）Autumn lamb embryo transfer pregnancy rate was significantly higher than that in spring（51.33% vs 26.07%,P〈0.05）.6）The transplantation of 2-8cells or blastocyst had no effect on pregnancy rate（P〉0.05）.Conclusion：The Suffolk lambs aged 5-6weeks,weighed 8-18 kg,with no history disease,can significantly improve the efficiency of follicular development.Autumn lamb developmental capacity of oocytes was significantly higher than that in spring.In the same season,there was no difference between the transplanted embryonic or blastocyst stage embryo conception rate.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2015年第4期141-146,共6页
Journal of China Agricultural University
基金
国家自然基金资助项目(U1203381)
新疆自治区科技计划项目(201111113)
新疆自治区科技支疆项目(201291147)
国家转基因重大专项(2014ZX08008-003)
山西省生态站项目
关键词
外源激素
萨福克羔羊
卵母细胞
体外受精
胚胎移植
exogenous hormones
Suffolk lamb
oocytes
in vitro fertilization
embryo transfer
