摘要
目的观察亚最低杀菌浓度(minimum bactericidal concentration,MBC)阿莫西林克拉维酸钾诱导对金黄色葡萄球菌标准菌株的耐药性影响。方法以金黄色葡萄球菌标准菌株ATCC 29213为研究对象,阿莫西林克拉维酸钾为抗菌药物,以微量稀释法定量检测不同诱导天数MBC值,采用1/2 MBC浓度对金黄色葡萄球菌进行35 d多步体外诱导试验,观察金黄色葡萄球菌标准菌株MBC变化并记录,全自动微生物药敏鉴定仪对最终诱导后菌株进行阿莫西林克拉维酸钾耐药性分析。结果金黄色葡萄球菌标准菌株MBC值在阿莫西林克拉维酸钾体外诱导10 d就明显升高,诱导35 d升高至原始菌株的32倍;诱导出的金黄色葡萄球菌耐药菌株对阿莫西林克拉维酸钾的耐药率为100%。结论亚MBC阿莫西林克拉维酸钾可体外诱导金黄色葡萄球菌标准菌株出现耐药,且耐药性随亚MBC诱导时间延长而增加,提示合理选择抗菌药物使用剂量可作为预防细菌耐药重要措施。
Objective To observe the effect of inducing by sub-MBC（ minimum bactericidal concentration） of Amoxicillin and Clavulanate Potassium on the antibiotic resistance of standard strains of staphylococcus aureus. Methods The standard strain of staphylococcus aureus ATCC 29213 and Amoxicillin and Clavulanate Potassium were respectively used as observational strain and antibacterials,and MBC values of different induced days were quantitatively detected using microdilution method. Induction test of staphylococcus aureus in vitro was performed using 1 /2 concentration of MBC for 35 d,and MBC changes were observed and recorded. The final antibiotic resistance to Amoxicillin and Clavulanate Potassium of staphylococcus aureus was identified using automatic microorganisms drug sensitivity analyzer. Results The MBC value of the standard strain of staphylococcus aureus was significantly increased which was induced by Amoxicillin and Clavulanate Potassium in vitro for 10 d,and the value induced for 35 d was increased to 32 times of original strain value. The induced drug-resistant strain of staphylococcus aureus had 100% resistance rate to Amoxicillin and Clavulanate Potassium. Conclusion The sub-MBC of Amoxicillin and Clavulanate Potassium can induce antibiotic resistance of the standard strains of staphylococcus aureus in vitro,and the degree of the antibiotic resistance increases with the increasing induction time by sub-MBC. A rational dosage of antimicrobial is important to prevent antibiotic resistance.
出处
《解放军医药杂志》
CAS
2015年第7期85-88,共4页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
江西省卫生厅资助课题(20092108)
