摘要
目的:研究比较不同趋化因子[骨髓基质细胞衍生因子1(stromal cell-derived factor-1,SDF-1)、碱性成纤维细胞生长因子(basic fibroblast growth factor,b FGF)和白介素8(interleukin-8,IL-8)]在多个浓度下对骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)的趋化作用。方法:实验分为3组,即SDF1组、b FGF组、IL-8组,每组因子浓度为0(对照浓度)、10、50、100、200、400 ng/m L(实验浓度)。抽取SD大鼠骨髓,分离培养BMSCs,利用Transwell小室研究不同浓度SDF1、b FGF、IL-8对BMSCs的趋化作用,获得3种因子趋化BMSCs的最佳浓度。采用SPSS16.0软件包对数据进行统计学分析。结果:与0 ng/m L(对照浓度)组相比,3种因子均可趋化BMSCs(P<0.05);50、100、400 ng/m L浓度下,SDF1对BMSCs的趋化作用显著高于b FGF(P<0.05);200 ng/m L浓度下,SDF1对BMSCs的趋化作用强于IL-8;SDF1及IL-8对BMSCc趋化作用的最佳浓度均为100 ng/m L,b FGF的最佳浓度为200 ng/m L。结论:SDF-1、b FGF、IL-8均可趋化BMSCs,SDF-1与IL-8的趋化效果更佳。
PURPOSE： To investigate chemotaxis of different concentrations of chemokines stromal cell-derived factor-1 （SDF-1）, basic fibroblast growth factor （bFGF） and interleukin-8 （IL-8）on bone marrow mesenchymal stem cells （BMSCs）. METHODS： The experiment group was divided into 3 groups according to different culture media： SDF1, bFGF and IL-8. The chemokine concentrations of each group was 0 ng/mL （control concentration）, 10 ng/mL, 50 ng/mL, 100 ng/mL, 200ng/ mL and 400 ng/mL （experimental concentration）. BMSCs were isolated from SD rats and cultured in vitro. 24-well plates with an 8 μm pore size polycarbonate membrane were used to examine the chemotaxis of SDF1, bFGF, IL-8 on BMSCs, respectively, and to obtain the best chemotactic concentrations of 3 chemokines. The data was analyzed with SPSS 16.0 software package. RESULTS： Compared with 0 ng/mL group, all chemokines could ehemoattract BMSCs （P〈0.05）. At the concentration of 50 ng/mL, 100 ng/mL and 400 ng/mL, SDF1 significantly recruited more cells than bFGF （P〈O.05）; SDF1 significantly recruited more cells than IL-8 at the concentration of 200 ng/mL; The best chemotaetie concentration was 100 ng/mL for SDF1 and IL-8 and 200 ng/mL for bFGF. CONCLUSIONS： The use of SDFI,bFGF,IL-8 can ehemoattraet BMSCs. The ehemotaxis of SDF1 and IL-8 are better than bFGF.
出处
《上海口腔医学》
CAS
CSCD
2015年第3期275-279,共5页
Shanghai Journal of Stomatology
基金
上海市科学技术委员会医学引导项目(124119b0101)~~
