摘要
目的：探讨人肝细胞癌（hepatocellularcarcinoma，HCC）组织中抑癌基因053蛋白、多药耐药基因P-糖蛋白（P—glycoprotein，P—gh）的表达，并进一步探讨两者表达强度之间的相关性及其与肝细胞癌临床病理参数之间的关系。方法：选取我院及西安交通大学第一附属医院2011年3月至2015年3月手术切除HCC组织标本60例作为实验组，同期住院的因外伤至肝破裂行手术切除的正常肝组织标本40例作为对照组。用免疫组化法检测肝脏组织中P-gh、p53的表达水平。结果：①实验组及对照组的肝脏组织中均有P—gh、p53蛋白不同程度的阳性表达，且在实验组中的阳性表达高于对照组，差异有统计学意义（P〈0．05）；②肝细胞癌中p53蛋白在不同的病理分级以及有无肿瘤胞膜的阳性表达存在差异，差异有统计学意义（P〈0．05）；肝细胞癌中P—gP蛋白在不同的病理分级、肿瘤大小以及有无肿瘤胞膜和门脉血栓的阳性表达存在差异，且差异有统计学意义（P〈0．05）。⑧p53、P—gP蛋白在肝细胞癌中的表达呈正相关性（P〈005）。结论：①人肝细胞癌组织中p53、P-gP蛋白的表达可能参与了肝细胞癌的发病；②人肝细胞癌组织中p53、P—gP蛋白的表达还可能是肝细胞癌部分生物学特性的重要指标之一。为临床上预防HCC复发、转移和预测化疗疗效、判断预后等提供参考依据。
Objective To explore the expression of repress oncogene p53 protein and P - glycoprotein, P - gh in hepatocellular carcinonm, HCC tissue. Have a further resear~＇h on correlation of the expression intensity among the three and their relationship with the pathological parameters of hepatocellular carcinoma. Methods subject investigated ： 60 cases of HCC resection tissues（ the experimental group） from March 2011 to March 2015 were selected from Hepatobiliary Surgery of the First Affiliated Hospital of Xi＇an Jiao Tong University. 40 ca- ses of normal liver tissues（ the control group） of Patients with Liver rupture who received the hepatectomy at the saare period of hospitalization. All the cases haven＇ t received Chemo- therapy and Radiotherapy before operation ,and all the tissues have received histopathologie examinations. Results： in liver tissue of the experimental group and control group P - gh, p53 protein positive expression of different level, and positive expression in the experimental group was higher than the control group; the p53 protein in hepatocellular carcinoma （HCC） in different pathological grading and presence of positive expression of tumor cell membrane is different; P - g＇p protein in bepatocellular carcinoma （HCC） in different pathologic stage, tumor size mad presence of tumor cell membrane and differences between the positive expression of portal vein thrombosis. Conclusion：（1）The expression of p53 and P - gp protein in human Hepatocenular carcinoma tissues might participate in the disease of Hepatocellular carcinoma. （2）The expression of p53 and P - gp protein in human Hepatoeellular carcinoma tissues might also he one of the important indexes of part of Biological Characteristics. For the prevention of HCC recurrence and metastasis and clinical prediction provided a reference for chemotherapy curative effect, prognosis, etc.
出处
《医学与社会》
2015年第B06期19-20,共2页
Medicine and Society
关键词
肝细胞癌
P-53
P-糖蛋白
hepatocellular carcinoma
P - 53
P - glycoprotein
