摘要
目的用全骨髓贴壁分离法分离培养骨髓间充质干细胞,通过建立原发性骨质疏松性骨折模型,探讨骨髓间充质干细胞对骨质疏松性骨折愈合情况。方法无菌条件下采集双侧股骨、胫骨骨髓,用全骨髓贴壁分离法培养出原代骨髓间充质干细胞,选取11周雌性SD大鼠60只,卵巢切除术(OVX)建立骨质疏松模型后,均做双侧骨折模型,随机分成对照组(OVX骨折不处理组)、生理盐水组(OVX骨折+生理盐水组)和细胞治疗组(OVX骨折+骨髓间充质干细胞治疗组),术后于第3天、第4、8周分别进行X-光扫描。结果全骨髓贴壁法成功分离出骨髓间充质干细胞,经流式细胞仪分析,培养出来的细胞与骨髓间充质干细胞表面抗原一致。X-光结果表明,细胞治疗组相比于其它组能加快骨质疏松性骨折愈合。结论体外分离大鼠骨髓间充质干细胞对原发性骨质疏松性骨折愈合有促进作用。
Objective To assess the therapeutic effect of bone marrow-derived mesenchymal stem cells（BMSCs）on osteoporotic fracture healing.Methods Bone marrow of the rat bilateral femur and tibia bone tissue were collected,and BMSCs were isolated by the whole bone marrow adherence method.Sixty female 11-week-old SD rats were ovariectomized（OVX）to induce osteoporosis followed by bilateral fracture generation.Twenty rats were left without giving any further treatment（OVX controls）,20 received injection of saline（OVX＋placebo control）and 20 were given injection of BMSCs（OVX＋BMSCs）.X-ray scan was performed at 3-day,4-week and 8-week post-fracture,respectively.Results Flow cytometry analysis revealed that the isolated BMSCs express surface antigens similar to those reported previously.X-ray results showed that compared with OVX and OVX＋placebo groups,BMSCs treatment markedly accelerated fracture healing of in osteoporotic rats.Conclusion Transplantation of BMSCs can effectively improve the healing of primary osteoporotic fracture.
出处
《国际检验医学杂志》
CAS
2015年第14期1984-1985,1987,共3页
International Journal of Laboratory Medicine
基金
广东省自然科学基金项目(S2013020012866)
广东医学院科研基金面上培育项目(M2014048)
湛江市科技创新能力建设专项项目(2014A501)
广东省医学科研基金(B2014313)
湛江市科技计划项目(2014A06005)
关键词
骨髓间充质干细胞
骨质疏松
骨折
Bone mesenchymal stem cells
Osteoporosis
Fracture
