摘要
目的观察中医不同治法对骨质疏松症小鼠骨密度(BMD)、碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)变化的影响,探讨中医防治骨质疏松症的机制。方法除正常组外,将骨保护素(OPG)基因敲除小鼠随机分为模型组、补肾组、健脾组、活血组和福善美组。检测各组小鼠BMD及血清ALP、TRAP含量。结果 1.与正常组比较,模型组小鼠BMD显著降低(P<0.05);与模型组比较,补肾组与健脾组BMD显著升高(P<0.05)。2.与正常组比较,模型组小鼠血清ALP含量显著升高(P<0.05);与模型组比较,补肾组、健脾组和福善美组小鼠血清ALP含量显著降低(P<0.05)。3.与正常组比较,模型组小鼠血清TRAP含量显著升高(P<0.05);与模型组比较,补肾组与健脾组小鼠血清TRAP含量显著降低(P<0.05)。结论补肾和健脾方法能显著提高骨质疏松小鼠的骨量,明显抑制成破骨活性,使小鼠脱离高骨转换状态,起到治疗骨质疏松症的作用。
Objective To observe the effect of different traditional Chinese medicine on BMD,ALP,and TRAP in osteoporotic mice,and to explore the mechanism of TCMin the treatment of osteoporosis. Methods Except the WT group,OPG knockout mice were randomly divided into model group,tonifying kidney group,invigorating spleen group,promoting blood flowgroup,and Fosamax group. BMD and the content of ALP and TRAP in the serum of the mice in different groups were detected. Re sults 1）BMD in the model group was significantly lower than that in the WT group（ P〈0. 05）. BMD in the tonifying kidney group or the invigorating spleen group was significantly higher than that in the model group（ P〈0. 05）. 2） The serum content of ALP in the model group was significantly higher than that in the WT group（ P〈0. 05）. The serum content of ALP in the tonifying kidney group,the invigorating spleen group,or the Fosamax group was significantly lower than that in the model group（ P〈0. 05）. 3）The serum content of TRAP in the model group was significantly higher than that in the WT group（ P〈0. 05）. The serum content of TRAP in the tonifying kidney group or the invigorating spleen group was significantly lower than that in the model group（ P〈0. 05）. Conclusion The tonifying kidney and invigorating spleen treatment can treat osteoporosis bye significantly improving bone mass,inhibiting osteoblast and osteoclast activities,and preventing from high bone turnover status in osteoporotic mice.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2015年第7期789-791,共3页
Chinese Journal of Osteoporosis
基金
辽宁省教育厅高校杰出青年学者成长计划(LJQ2012084)
