摘要
目的:比较荧光定量PCR(real-time PCR)与普通反转录酶-聚合酶链锁反应(RT-PCR)对人偏肺病毒(hMPV)的检测价值。方法:Real-time PCR和RT-PCR同时对500例急性下呼吸道感染(ALRTI)患儿鼻咽部分泌物进行h MPV检测,分析两种方法的特异性和灵敏性。结果:Real-time PCR和RT-PCR的检出率分别为16%及9.8%,RT-PCR与Real-time PCR比较,灵敏度为31.3%(25/80),特异度为94.3%(396/420)。结论:Real-time PCR检测hMPV敏感性高于RT-PCR,是临床检测儿童鼻咽部分泌物h MPV感染的有效的方法。
Objective：To investigate the effect of real-time quantitative PCR and RT-PCR in detec-tion of human metapneumo virus. Methods：Real-time PCR and RT-PCR are adopted to test hMPV on throat exudate of 500 acute lower respiratory tract infection（ ALRTI）child patients,analyzing the spe-cificity and sensitivity of both assays. Results：Comparing Real-time PCR and RT-PCR,the detection rates were 16 % to 9. 8%. Comparing Real-time PCR and RT-PCR,the sensitivity and specificity were 31. 3 %（25/80）and 94. 3%（396/420）respectively. Conclusion：The sensitivity of Real-time PCR is significantly higher than RT-PCR in detection of hMPV,which provid an effective method for detection of hMPV.
出处
《贵阳医学院学报》
CAS
2015年第8期834-837,840,共5页
Journal of Guiyang Medical College
基金
2013年贵阳市科技局计划基金项目[筑科合同(2013103)31号]
关键词
实时荧光定量PCR
逆转录聚合酶链反应
儿童
感染
人偏肺病毒
real-time polymerase chain reaction
reverse transcription polymerase chain reaction
child
infection
human metapneumo virus
