摘要
目的探讨HPLC法测定小儿肺热咳喘口服液中苦杏仁苷含量的可行性。方法采用高效液相色谱法测定，Thermo-C18柱（250×4．6mm，5μm），甲醇一水（13：87）为流动相，流速为1．0ml／min，检测波长为196nm。结果苦杏仁苷在0．1118～1．677μg间具有良好的线性关系（r^2=0．999），苦杏仁苷平均回收率为99．77％，RSD为0．424（n=6）。结论HPLC法测定小儿肺热咳喘口服液中苦杏仁苷含量简便、准确、重现性好，可用于小儿肺热咳喘口服液中苦杏仁苷的质量控制。
Objective To eValuate the feasibility of determination of Laetrile in Infantile Feirekechuan oral liquid by HPLC. Methods Using high performance liquid chromatography （HPLC） method, Thermo-C18 column （250 × 4.6 mm, 5 μm）, methanol-water （13：87） as mobile phase, flow rate of 1.0 ml/min, detection wavelength of 196 nm. Results As lae- trile in μg 0. 1118 -0. 1118 has a good linear relationship between μg （r2 = 0. 999）, laetrile the average recovery was 99. 77%, RSD was 0. 424 （n = 6）. Conclusion Determination of Laetrile in Infantile Feirekechuan oral liquid by HPLC is simple, accurate, reproducible.
出处
《临床合理用药杂志》
2015年第23期37-39,共3页
Chinese Journal of Clinical Rational Drug Use
