摘要
强直性脊柱炎（ankylosing spondylitis,AS）是临床常见的一种自身免疫性疾病。90%~95%的AS患者有人类白细胞抗原B27（human leucocyte antigen B27,HLA-B27）基因的表达,说明HLA-B27与AS有高度关联性。因此,HLA-27的检测在AS的诊断中有很重要的诊断价值和临床意义。目前用于检测HLA-B27的实验室方法主要有微量细胞毒法（microlymphocytotoxic test,MLCT）、酶联免疫吸咐法（enzyme linked immunosorbent assay,ELISA）、流式细胞仪法（flow cytometry instrument method,FCM）、PCR等。本文将对AS的这些实验室诊断方法及其优缺点予以概述,为实验室根据自身条件选择合适的方法检测AS提供一个参考依据。
Ankylosing spondylitis（AS） is a common clinical autoimmune disease. There are 90%~95% of the patients with AS showed the expression of HLA-B27 gene, which suggest that HLA-B27 is strongly associated with AS. Therefore, the detection of HLA-27 has very important diagnostic value and clinical significance for the diagnosis of AS. Nowadays, the methods used for detecting the HLA-B27 include microlymphocytotoxic test（MLCT）, enzyme-linked immunosorbent assay（ELISA）, flow cytometry assay（FCM）and PCR. In this paper, the current methods for the laboratory diagnosis of AS will be reviewed to provide a reference for laboratory to select the appropriate method based on the detection of AS according to its own conditions.
出处
《分子诊断与治疗杂志》
2015年第4期276-280,共5页
Journal of Molecular Diagnostics and Therapy
