摘要
目的探讨荧光原位杂交检测乳腺浸润性导管癌人类表皮生长因子受体-2(HER-2)基因表达及其与特异AT序列结合蛋白1(SATB1)、激素受体(HR)蛋白表达关系。方法选取乳腺浸润性导管癌手术患者72例,术中采集乳腺癌组织标本,免疫组化法(IHC)检测HER-2、SATB1和HR蛋白表达,采用荧光原位杂交法(FISH)检测HER-2基因扩增。结果 FISH检测HER-2基因扩增率为38.89%(28/72),IHC检测HER-2蛋白阳性率为22.22%(16/72),两组比较差异有统计学意义(P<0.05);HER-2基因扩增(+)组SATB1阳性率明显高于HER-2基因扩增(-)组(P<0.05),HR阳性率明显低于HER-2基因扩增(-)组(P<0.05)。经Pearson相关性分析,HER-2基因扩增与HR蛋白表达呈负相关(r=-0.405,P<0.05),与SATB1蛋白表达呈正相关(r=0.396,P<0.05);HER-2基因扩增与组织学分级、淋巴结转移和TNM分期紧密相关,其中组织学Ⅲ级和临床Ⅲ期者HER-2基因扩增阳性率明显高于组织学Ⅰ、Ⅱ级和临床Ⅰ、Ⅱ期,有淋巴结转移者HER-2基因扩增阳性率明显高于无淋巴结转移者(P<0.05)。HER-2基因扩增与年龄、肿瘤直径大小无明显相关性(P>0.05)。结论荧光原位杂交检测HER-2基因扩增对乳腺浸润性导管癌诊断率高,且与SATB1、HR蛋白表达紧密相关,三者可预示临床分期和淋巴结转移特征。
Objective To explore gene expression of human epidermal growth factor receptor 2（ HER- 2） in invasive breast ductal carcinoma detected by fluorescence in situ hybridization（ FISH） and its relationship with special AT- rich sequence binding protein（ SATB1） and hormone receptor（ HR） proteins expressions. Methods Seventy- two patients with invasive breast ductal carcinoma were selected,the tissue specimens were obtained during operation,immunohistochemistry was used to detect the expressions of HER- 2,SATB1,and HR proteins; FISH was used to detect HER- 2 gene amplification. Results The amplification rate of HER- 2 gene detected by FISH was 38. 89%（ 28 /72）,the positive rate of HER- 2 protein detected by IHC was 22. 22%（ 16 /72）,there was statistically significant difference between the two groups（ P〈0. 05）; the positive rate of SATB1 in HER- 2 gene positive amplification group was statistically significantly higher than that in HER- 2 gene negative amplification group（ P〈0. 05）,the positive rate of HR in HER- 2 gene positive amplification group was statistically significantly lower than that in HER- 2 gene negative amplification group（ P〈0. 05）. Pearson correlational analysis showed HER- 2 gene amplification was negatively correlated with HR protein expression（ r =- 0. 405,P〈0. 05） and positively correlated with SATB1 protein expression（ r = 0. 396,P〈0. 05）; HER- 2 gene amplification was closely correlated with histological grading,lymph node metastasis,and TNM staging; the positive rates of HER- 2 gene amplification in patients of histological grade Ⅲ and clinical stage Ⅲ were statistically significantly higher than those in patients of histological grade Ⅰ ＋ Ⅱ and clinical stage Ⅰ ＋ Ⅱ,the positive rate of HER- 2 gene amplification in patients with lymph node metastasis was statistically significantly higher than that in patients without lymph node metastasis（ P〈0. 05）; there was no significant correlation between HER- 2 gene amplification and age,the diameter of tumor（ P〉0. 05）. Conclusion The diagnostic rate of HER- 2 gene amplification by FISH in invasive breast ductal carcinoma is high,and it is closely related to SATB1 and HR proteins expressions,all the three indexes can predict clinical stages and lymph node metastasis.
出处
《中国妇幼保健》
CAS
2015年第22期3884-3887,共4页
Maternal and Child Health Care of China
