摘要
以蓝莓根际土壤中筛选出的纺锤芽孢杆菌(Bacillus fusiformis)作为主要菌株,复配成蓝莓PGPR液体菌剂(P),以发酵鸡粪(OF)为吸附载体制成生物有机肥(PF),研究了PF、P和OF对蓝莓根际土壤微生物特性及土壤肥力的影响。结果表明,施肥可改善土壤化学性质和微生物学特性,但不同处理之间差异很大。同P处理相比,PF处理的碱解氮、有效磷、有机质和全氮含量显著增加23.18%,21.72%,71.74%和11.26%。施肥同样对蓝莓根际土壤的有机碳及其组分具有很大影响,虽然PF处理对富里酸含量影响不显著,但显著增加了根际土壤中有机碳的含量,提高了胡/富。PF、P和OF可导致土壤细菌和微生物总量显著降低,但对微生物量碳/氮比的影响差异不显著。同P和OF处理相比,PF处理的微生物量碳显著增加65.52%和54.84%。此外,同对照相比,施肥显著提高蔗糖酶和酸性磷酸酶活性,但对过氧化氢酶、脲酶和多酚氧化酶活性影响不大。其中,PF的酸性磷酸酶活性显著高于P处理。主成分分析结果表明,不同施肥处理土壤质量的次序为PF>OF>P>CK。综合以上分析,以纺锤芽孢杆菌为主的微生物肥料可不同程度的提高蓝莓根际土壤的生物活性和土壤肥力,但以生物有机肥的形式施用效果最佳。
The main strain, Bacillus fusiformis, was selected from rhizophere soil of Blueberry by biological assay in order to make a plant growth-promoting rhizobacteria（PGPR） liquid inoculants（P） together with other strain. PGPR biological organic fertilizer （PF） was made by decomposed chicken manure （OF） adsorbing the liquid inoculants（P）. The effects of PF, P and OF on microbial properties and soil fertility in Blueberry rhizophere soil were studied. The results showed that fertilization（PF, OF and P） improved soil chemical properties and microbiological properties, but there were great differences among PF, OF and P treatment. Compared with P treatment, PF could significantly increase alkali-hydrolyzable nitrogen, available phosphorus, organic matter and total nitrogen by 23.18%, 21.72%, 71.74% and 11.26%, respectively. Fertilization had a strong influence on rhizosphere soil organic carbon and its fractions. PF had no significant influence on fulvic acid, but increased significantly organic carbon and HA/FA in rhizophere soil. PF, P and OF could significantly decrease the quantity of bacteria and total microorganism populations, and had no significant effect on MBC/MBN. Compared with P and OF, PF could significantly increase soil microbial biomass C（MBC） by 65.52% and 54.84%. In addition, compared with CK, fertilization increased significantly invertase activity and acid phosphatase activity, but had no influence on the catalase, urease and polyphenol oxidase activity. Moreover, acid phosphatase activity was significantly higher in PF than in P. Based on principal component analysis, soil quality in different treatments listed as follow： PF〉OF〉P〉CK. In conclusion, microbial fertilizer, which mainly contained Bacillus fusiformis, could improve soil bioactivity and soil fertility to different extent, and application of biological organic fertilizer showed the best improvement.
出处
《水土保持学报》
CSCD
北大核心
2015年第3期167-171,177,共6页
Journal of Soil and Water Conservation
基金
国家林业公益性行业科研专项(201204402)
农业科技成果转化资金项目"经济林新型生物肥料关键技术中试与示范"
关键词
生物肥
土壤微生物特性
土壤肥力
蓝莓
bio-fertilizer
soil microbial properties
soil fertility
blueberry
