摘要
背景:临床上便秘患者常常伴有抑郁情绪,抑郁患者也常常伴有便秘症状,其机制不清。目的:通过建立动物模型了解便秘和抑郁的关系。方法:将60只SPF大鼠随机分成3组:正常组、慢性应激抑郁模型组和慢传输便秘模型组。慢传输便秘模型组大鼠每日给予复方地芬诺酯灌胃,给药剂量为8 mg/kg,持续时间120 d。实验第100天开始随机予慢性应激抑郁模型组不可预见刺激21 d。正常组大鼠常规饲养。每周记录1次大鼠粪便粒数、粪便干质量及大鼠体质量。建模成功后检测各组大鼠粪便粒数、粪便干质量、首粒黑便排出时间、糖水偏爱百分比、旷场实验行为学得分、强迫游泳时间并进行统计分析。结果与结论:慢性应激抑郁模型组粪便粒数较正常组少(P=0.00)、首粒黑便排出时间较正常组长(P=0.00);粪便粒数较慢传输便秘模型组多(P=0.00),首粒黑便排出时间较慢传输便秘模型组短(P=0.00)。慢传输便秘模型组的液体消耗量、糖水偏爱百分比、旷场实验水平得分和垂直得分、强迫游泳时间均较正常组均差异无显著性意义(P>0.05),但均高于慢性应激抑郁模型组(P<0.01)。结果证实,实验成功建立的慢性应激抑郁模型大鼠可表现出便秘现象,而慢传输便秘模型大鼠未能表现出抑郁现象,表明抑郁可以导致便秘,但便秘不一定会导致抑郁。
BACKGROUND： Clinically, patients with constipation often have depression and depression is often associated with symptoms of constipation, but the mechanisms remain unclear. OBJECTIVE： To observe the relation between constipation and depression by establishing animal models. METHODS： A total of 60 specific-pathogen-free rats were divided randomly into three groups： normal group, chronic stress-induced depression model group and slow transit constipation model group. Slow transit constipation model was created by giving the solution of compound diphenoxlate 8 mg/kg daily, for 120 consecutive days. On day 100, chronic stress model rats were subjected to successive unforeseen stimulus for 21 days. Rats in the normal group were regularly housed. Fecal number, dry weight of feces and body weight were recorded once a week. After successful model establishment, fecal number, dry weight of feces, time of first dark stools defecation, sucrose preference, open-field behavior score and forced swim time were detected in each group, and statistically analyzed.RESULTS AND CONCLUSION： Fecal number was less in the chronic stress-induced depression model group than in the normal group（P = 0.00）, and time of first dark stools defecation was longer（P = 0.00）. Fecal number was more in the chronic stress-induced depression model group than in the slow transit constipation model group（P = 0.00）, but time of first dark stools defecation was shorter（P = 0.00）. No significant difference in fluid consumption, sucrose preference, horizontal and vertical scores of open-field test, and forced swim time was detected between slow transit constipation model group and normal group（P〈0.05）. However, above indexes were higher in the slow transit constipation model group than in the chronic stress-induced depression model group（P〈0.01）. Results verified that constipation was observed in chronic stress-induced rat model, but depression was not observed in slow transit constipation rat model, suggesting that depression can induce constipation, but constipation does not necessarily lead to depression.
出处
《中国组织工程研究》
CAS
北大核心
2015年第27期4356-4360,共5页
Chinese Journal of Tissue Engineering Research
基金
四川省2011年第五批科技计划项目(成财教【2011】366号)~~
