摘要
目的：建立滇重楼生长年限的鉴别方法,为滇重楼的质量控制提供参考。方法：利用傅里叶变换红外光谱技术测试了同一产地7种不同生长年限的68株重楼主根木质部和表皮样品的红外光谱,利用Omnic8.0软件计算样品的二阶导数光谱和四阶导数光谱,分别以主根木质部和表皮在1 800~900 cm-1的红外光谱、二阶导数红外光谱和四阶导数红外光谱数据为样品特征,采用Mahalanbis距离逐步判别分析法,依据Fisher线性判别准则建立重楼生长年限的判别模型,对未知生长年限的样品进行鉴别比较。结果：四阶导数光谱比其他级别的光谱鉴别效果好,表皮光谱比木质部光谱的鉴别效果好;利用表皮的四阶导数光谱数据建立判别分析模型对重楼的生长年限进行鉴别时,训练样本回判正确率100%,测试样本预测正确率78.6%,总正确率95.6%。结论：傅里叶变换红外光谱结合逐步判别分析法可鉴别重楼的生长年限,为生产实践中预测重楼的生长年限提供了一种新思路。
Objective： To establish a method to identify growing years of Paridis Rhizoma,in order to provide basis for quality control of this plant. Method： A total of 68 epidermises and xylems of taproot samples with seven different growing years of Paridis Rhizoma from the same origin was analyzed by Fourier-transform infrared（ FTIR） spectroscopy. The second derivative spectra and fourth derivative spectra of these tested samples were obtained by Omnic8. 0 software. With the infrared spectra, the second derivative spectra and the fourth derivative spectra of epidermises and xylems of taproot samples ranging between 1 800 and 900 cm-1as the sample characteristics,the discriminant model of growing years of Paridis Rhizoma was established by Mahalanbis distance gradual discriminant analysis method on the basis of Fisher linear discriminant criterion,in order to identify and compare these tested samples with unknown growing years. Result： The fourth derivative spectra had a better discrimination effect than other types of infrared spectra,while epidermises samples had a better discrimination effect than xylems samples. Moreover, the discriminant analysis model was established based on the fourth derivative spectra of epidermises to identify growing years of Paridis Rhizoma,with the return discriminant accuracy of training samples of 100%,the predicted accuracy of tested samples of 78. 6% and a total accuracy of 95. 6%.Conclusion： FTIR combined with gradual discriminant analysis can be utilized to identify growing years of Paridis Rhizoma and provide a new method for prediction of growing years of Paridis Rhizoma in the production practice.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第15期35-39,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81460584)
云南省大学生创新创业训练计划项目(201411390003)
关键词
红外光谱
判别分析
重楼
生长年限
木质部
表皮
infrared spectroscopy
discriminant analysis
Paridis Rhizoma
growing years
xylem
epidermises
