摘要
目的：建立防风通圣丸中栀子苷、连翘苷、5-O-甲基维斯阿米醇苷、黄芩苷、黄芩素、汉黄芩素、甘草酸铵7个成分的高效液相色谱测定方法。方法：采用phenomenex Luna 5u-C18色谱柱（4.6 mm×250 mm,5μm）,流动相甲醇（A）-0.1%磷酸溶液（B）,梯度洗脱,流速0.8 m L·min-1,检测波长分别为234,254 nm。结果：栀子苷、连翘苷、5-O-甲基维斯阿米醇苷、黄芩苷、黄芩素、汉黄芩素、甘草酸铵7个成分在相应的范围内线性关系良好,r不低于0.999 1;平均回收率97.3%~99.8%;RSD均〈2.0。结论：经方法学验证,本方法简单、快速、灵敏、准确,可用于防风通圣丸中栀子苷、连翘苷、5-O-甲基维斯阿米醇苷、黄芩苷、黄芩素、汉黄芩素、甘草酸铵7个成分的含量测定。
Objective： To develop an HPLC method for simultaneous determination of geniposide,phillyrin,5-O-methylvisammioside,baicalin,baicalein,wogonin,and ammonium glycyrrhizinate in Fangfeng Tongsheng pill. Method： The chromatographic separation was on phenomenex Luna 5u-C18（ 4. 6 mm × 250 mm,5 μm） with a mobile phase consisting of methanol（ A）-0. 1% phosphoric acid solution（ B） in a gradient mode at a flow rate of 0. 8 m L·min-1. The detection wavelengths were 234 nm and 254 nm. Result： Within the linear range of geniposide,phillyrin,5-O-methylvisammioside,baicalin,baicalein,wogonin,ammonium glycyrrhizinate,the correlation coefficients were lager than 0. 999 1. The average recoveries of the seven components were in the range of 97. 3%-99. 8%. RSD were less than 2. 0%. Conclusion： By method validation,this method is proved to be simple,rapid,sensitive and accurate. It can be used to determine the contents of geniposide,phillyrin,5-Omethylvisammioside,baicalin,baicalein,wogonin,and ammonium glycyrrhizinate in Fangfeng Tongsheng pill.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第15期79-83,共5页
Chinese Journal of Experimental Traditional Medical Formulae
