摘要
目的建立鉴定A型肉毒毒素的ELISA鉴别试验方法以替代传统的动物试验法。方法采用现代免疫学技术,制备马源性和兔源性抗A型肉毒毒素多克隆抗体,建立了双抗体夹心ELISA,并就此初步进行方法学验证。结果所建立的ELISA具有良好的特异性、灵敏度、精密度和耐用性,具有替代动物试验方法的良好前景。结论在进一步验证和确认之后,该方法有望正式成为可用于鉴定A型肉毒毒素的试验方法以替代动物试验法。
Objective To establish a new identification method for botulinum toxin type A（ bot A） to replace the traditional animal test. Methods A double antibody sandwich ELISA was established based on the modern immunological technology,by using prepared two kinds of anti-bot A polyclonal antibodies derived from equine and rabbit origin,and a preliminary verification test was performed. Results The established identification ELISA could be a potential alternative method in replacement of animal test,it is of good specificity,sensitivity,precision and robustness. Conclusion The established method will be expected to be approved for the identification test of botulinum toxin type A as the replacement method for traditional animal test by a further verification and validation.
出处
《微生物学免疫学进展》
2015年第4期31-33,共3页
Progress In Microbiology and Immunology
