摘要
目的探讨S-烯丙基巯基半胱氨酸(SAMC)对裸鼠胃癌模型移植瘤的抑制作用和相关机制。方法用裸鼠皮下胃癌SGC7901细胞注射法建立移植瘤模型,模型制作成功后将模型鼠分为对照组、SAMC低剂量组、SAMC高剂量组、5-FU组,每组6只。干预处理21d后观察各组肿瘤大小并计算每组肿瘤的瘤重、抑制率、肿瘤微血管密度及凋亡指数,免疫组织化学法检测各组肿瘤组织内血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达情况。结果与对照组相比,SAMC低剂量组、SAMC高剂量组、5-FU组移植肿瘤重量明显降低,抑瘤率分别为29.89%、51.85%和53.17%;微血管密度SAMC低剂量组、SAMC高剂量组、5-FU组均比对照组明显减少,分别为(11.78±7.49)%、(7.82±5.19)%、(7.91±4.37)%VS(16.21±8.52)%;凋亡指数比对照组明显升高,SAMC低剂量组、SAMC高剂量组、5-FU组分别为(11.82±1.60)%、(14.93±1.68)%和(15.92±1.72)%VS(4.36±1.55)%;免疫组织化学结果示VEGF表达量SAMC低剂量组、SAMC高剂量组、5-FU组均比对照组明显减少,平均光密度值(IOD)分别为11 240±1150、7 535±856、7 820±650 VS 18 500±1 522。结论 SAMC可明显抑制裸鼠原位肿瘤的生长,其机制可能通过抑制肿瘤新生血管的生成而诱导胃癌细胞凋亡。
Objective To investigate the effect of SAMC to human gastric cancer implant tumors in nude mice and its relevant mechanism.Methods Model of human gastric cancer was established by orthotopic transplantation,then the nude mice were randomly divided into four groups of six mice each：SAMC low does group,SAMC high does group,control group and positive control group receiving 5-FU.Tumor size was measured and antitumor activity was calculated and apoptosis in the implanted tumor cells was manifested by the TUNEL method.Intratumoral microvessel density and vascular endothelial growth factor（VEGF）were manifested by immunohistochemistry analyses.Results The tumor volume inhibition rates were 29.89%,51.85% and 53.17% in SAMC low,high dose group and 5-FU group,respectively;Tumor volume of experimental group compared with control groups（P〈0.05）,with no signifiant difference between the high dose and 5-FU groups（P〉0.05）.TUNEL demonstrated apoptosis indices of11.82±1.60% and 14.93±1.68% for the SAMC low and high dose groups,15.92±1.72% for the 5-FU group and 4.36±1.55% for the control groups.Immunohistochemical staining demonstrated that SAMC treatment decreased intratumoral microvessel density and VEGF expression in implanted tumor when compared with the control group.Conclusions This study indicates that SAMC in vivo has strong inhibitory effects on human gastric cancer implant tumors in nude mice and may regulate intratumoral microvessel density to induce gastric cancer apoptosis.
出处
《中国煤炭工业医学杂志》
2015年第8期1352-1356,共5页
Chinese Journal of Coal Industry Medicine
基金
唐山市科技局科研计划项目(编号:130603)
