摘要
目的探讨活化白细胞黏附分子(activated leukocyte cell adhesion molecule,ALCAM)和癌胚抗原相关细胞黏附分子(carcinoembryonic antigen-related cell adhesion molecule,CEACAM)在非小细胞肺癌组织中的表达及与患者临床病理特征及预后的相关性。方法免疫组织化学及实时定量荧光聚合酶链反应(real-time,PCR)检测ALCAM、CEACAM-6在46例肺癌及癌旁组织中的表达;分析ALCAM,CEACAM-6及临床各指标与预后的关系。结果 ALCAM和CEACAM-6在癌组织中的表达明显高于癌旁组织;其中ALCAM在鳞癌组织中阳性表达率100%,在腺癌组织中阳性表达率56%,差异有统计学意义(P<0.05),且ALCAM,CEACAM-6在T1期患者肿瘤组织中的表达显著高于T2+T3期,差异有统计学意义(P<0.05)。ALCAM和CEACAM-6蛋白表达与肿瘤患者年龄、性别、淋巴结转移生存期差异无统计学意义(P>0.05)。ALCAM和CEACAM-6在非小细胞肺癌组织中呈现正相关,且阳性表达患者预后优于阴性表达患者。结论 ALCAM、CEACAM-6蛋白在非小细胞肺癌组织中高表达,并可能与患者预后相关。
Objective To investigate the expression levels of activated leukocyte cell adhesion molecule （ALCAM） and carcino-embryonic antigen related cellular adhesion molecule-6 （CEACAM-6） and their relationship with prognosis in patients with non-small cell lung cancer （NSCLC） as well as their clinicopathologic significance. Methods Immunohistochemical and RT-PCR methods were used to detect the expression of ALCAM and CEACAM-6 in 46 NSCLC specimens. The relationship between the expression of ALCAM and CEACAM 6 and clinicopathological features was statistically analyzed. Results The expression of both ALCAM and CEACAM-6 were significantly higher in NSCLC than in adjacent tissues, including 100% positive rate of ALCAM in squamous cell carcinoma and 56% positive rate of ALCAM in adenocareinoma （ P 〈 0.05）. How the expression of ALCAM and CEACAM-6 was significantly higher in stage T1 than in stage T2 and T3 （ P d0.05）. There were no significant correlation between the protein expression of ALCAM and CEACAM-6 and the age, gender, tumor classification, lymphatic metastasis, pathological stage or lifetime （ P 〉0.05）. A positive correlation of the expression of ALCAM with CEACAM-6 was found in NSCLC, and the prognosis of patients with NSCLC was significantly better in positive expression of ALCAM and CEACAM-6. Conclusion ALCAM and CEACAM-6 were highly expressed in NSCLC, and may correlate to prognosis.
出处
《临床荟萃》
CAS
2015年第8期870-874,共5页
Clinical Focus
基金
国家自然科学基金资助项目(81370372)
关键词
癌
非小细胞肺
活化白细胞黏附分子
细胞黏附分子
预后
carcinoma, non-small-cell lung
activated-leukocyte cell adhesion molecule
cell adhesionmolecules
prognosis
