摘要
目的分析前列腺特异性膜抗原（PSMA）及其新型剪接变异体（PSM-E）在预测前列腺癌患者生化复发上的作用。方法选取行前列腺癌根治术，影像学资料未提示转移而Gleason评分〉7分的局限性前列腺癌患者15例，荧光定量PCR检测PSM-E和PSMA在术前外周血及切出肿瘤组织中的RNA表达，分析外周血及组织中PSM-E和PSMA相对表达的差异。术后随访患者前列腺特异性抗原（PSA）的变化。结果PSM-E和PSMA在前列腺癌组织中表达高于外周血，差异具有统计学意义（P〈0．01）。在前列腺癌组织中，PSM-E的表达明显低于PSMA；在前列腺癌患者的外周血中，PSM-E的表达明显高于PSMA，差异具有统计学意义（P〈0．01）。外周血PSM-E和PSMA表达阳性的患者术后生化复发率为66．7％（8／12），远处转移率为25．0％（3／12），均高于表达阴性的患者（0．O％，0／3），3例远处转移者表现为骨转移。结论在前列腺癌患者中，外周血检测PSM-E与PSMA均能地作为预测前列腺癌生化复发的指标。
Objective To analyze the value of prostate-specific membrane antigen （PSMA） and its new splicing variant （PSM-E） in predicting the biochemical recurrence of prostate cancer. Methods 15 prostate cancer patients without metastasis indicated by imaging data and whose Gleason score 〉7 and undergoing radical prostatectomy were selected. The expressions of PSMA and PSM-E in peripheral blood and prostate cancer tissue were detected by real-time PCR before the operation. The differences of the expressions of PSMA and PSM-E between in peripheral blood and in prostate cancer tissue were analyzed. The changes of prostate specific antigen （PSA） in the patients after surgery were followed up. Results The expressions of PSMA and PSM-E was higher in tissue than in peripheral blood, with statistical differences （P〈0.01）. The expression of PSM-E in tissue was statistically lower but in peripheral blood was statistically higher than that of PSMA （P〈0.01）. The postoperative biochemical recurrence ratio was 66.7% （8/12） in the patients with positive expressions of PSM-E and PSMA in peripheral blood and the metastasis ratio was 25.0% （3/12）. Both of them were higher than in the patients with negative expressions whose ratio was 0.0% （0/3）. All the three metastasis patients were bone metastasis. Conclusions Both PSME and PSMA can predict the biochemical recurrence of prostate cancer through peripheral blood.
出处
《国际医药卫生导报》
2015年第15期2104-2107,共4页
International Medicine and Health Guidance News
基金
广东省科技计划项目（20098030801131）
关键词
前列腺特异性膜抗原
微转移
剪接变异体
生化复发
Prostate-specific membrane antigen
Micro-metastasis
Spliced variant
Biochemicalrecurrence
