摘要
目的:探讨薏苡仁土炒炮制前后甘油三油酸酯含量的变化。方法:采用高效液相蒸发散射法对薏苡仁不同炮制品中甘油三油酸酯进行含量测定;Diamonsil C18(5μm,250 mm×4.6 mm)色谱柱;乙腈∶二氯甲烷(65∶35)为流动相;柱温30℃;流速0.7 m L·min-1;载气流速1.4 L·min-1,漂移管温度60℃。理论塔板数按甘油三油酸酯峰计算应不低于5 000。结果:甘油三油酸酯含量薏苡仁土炒品0.76%高于薏苡仁生品0.54%。结论:薏苡仁经土炒炮制后甘油三油酸酯的含量较生品有明显提高,薏苡仁土炒后甘油三油酸酯的含量升高可能是薏苡仁功效变化的依据。
Objective：To study glycerol trioleate content changes of Yiyiren（Semen Coicis）before and after processing.Methods：Using HPLC ELSD method to determine the content in different processed products of Yiyiren;Diamonsil C1 8 （5 microns,250 mm ×4.6 mm）chromatographic column,Acetonitrile,dichloromethane （65 35 ） as mobile phase;column temperature 30 ℃,flow rate 0.7 mL·min -1 ;carrier gas flow rate,min 1 .4 L·min -1 ,drift tube temperature 60 ℃.Theoretical plate number calculated should be not less than 5 000 as three oleic acid glycerol ester peak.Results：The glycerol trioleate content of Fried product 0.76% was higher than that of Yiyiren 0.54%.Conclusion：Glycerol trioleate content after stir-baked with soil increased may be the basis of effect change.
出处
《中医学报》
CAS
2015年第7期1017-1020,共4页
Acta Chinese Medicine
基金
国家中医药管理局中医药行业科研专项(201207004-8
20110700701)
关键词
薏苡仁
炮制品
甘油三油酸酯
高效液相蒸发散射法
semen coicis
processed products
glycerol trioleate
high performance liquid chromatography
