摘要
采用PCR-STR及基因分型技术,对广西毛南族167名(女57,男110)健康无关个体4个X-STR基因座(DXS7133、DXS8378、DXS6789和DXS7423)的遗传多态性进行研究。结果显示4个X-STR基因座分别检出4、5、9、3个等位基因和5、9、18、5种基因型,4个X-STR基因座女性的基因型频率分布均符合Hardy-Weinberg平衡定律(P>0.05)。群体遗传多态性指标为:多态信息含量(PIC)0.9611、男性个体识别率(DPmale)0.9771、女性个体识别率(DPfemale)0.9980、父-母-女三联体非父排除率(MECtrio)0.9611、父-女二联体非父排除率(MECduo)0.8821,显示上述4个X-STR基因座均具有较高多态性,在法医学个人识别、亲权鉴定及群体遗传学研究中有重要应用价值,同时也为人类群体遗传学、法医学等研究提供了广西毛南族群体X-STR基因座的基础数据,丰富了中华民族基因数据库。
To determine the genetic polymorphism of four X-STR loci among 167 unrelated individuals （57 female, 110 male） for Maonan groups from Guangxi Province, DXS7133, DXS8378, DXS6789 and DXS7423 were genotyped by multiplex PCR and Genscan. The numbers of alleles in the four X-STR loci were 4, 5, 9 and 3, respectively; the numbers of genotypes in the four loci were 5, 9, 18 and 5, respectively. The genotype frequencies in females were in accordance with Hardy-Weinberg equilibrium （P〉0.05）. The combined polymorphisminformation content （PIC） was 0.9611, power of discrimination （DP） was 0.9771 in males and 0.0.9980 in females, and the combined mean exclusion chance （MEC） was 0.0.9611 in the trio cases and 0.8821 in the duo cases. The 4 X-STR loci were highly polymorphic in Guangxi Maonan population and it is powerful for individual identification, paternity testing and population genetics. As well as the human population genetics and forensic science research offers Guangxi Maonan group X-STR loci of basic data, enrich the genetic database of the Chinese nation.
出处
《人类学学报》
CSSCI
CSCD
北大核心
2015年第3期397-403,共7页
Acta Anthropologica Sinica
基金
广西自然科学基金项目(2010GXNSFA013164)
关键词
X染色体
短串联重复序列
遗传多态性
遗传距离
广西毛南族
X-chromosome
Short tandem repeats
Genetic polymorphism
Genetic distance
Guangxi Maonan ethnic group
