不同干预方法对血管性痴呆大鼠神经再生及脑组织N-myc蛋白表达的影响被引量：2

Effect of different interferences on expression of nerve regeneration and N-myc in cerebral tissues of vascular dementia rats

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摘要目的探讨不同干预方法对血管性痴呆大鼠神经再生及脑组织N-myc蛋白表达的影响。方法雄性Wistar大鼠实验随机分为4组:假手术组,模型组,训练组,b FGF组。采用两血管阻断加硝普钠降压法制作大鼠血管性痴呆(VD)动物模型,用HE、和免疫组织化学方法检测海马组织N-myc蛋白的表达及穿梭箱训练和b FGF的干预作用。结果随着脑缺血再灌注3 d缺血海马神经元N-myc阳性细胞明显增多,随缺血再灌注时间的延长逐渐增多,14 d达高峰。应用穿梭箱训练和b FGF的干预后N-myc阳性细胞明显增加。结论穿梭箱训练和b FGF促进神经再生及缺血脑组织N-myc蛋白的表达,提示穿梭箱训练和b FGF促进神经再生作用可能由N-myc信号介导。 Objective To investigate the expression of N-myc expression and hippocampal neurogenesis in a rat model of cerebral ischemia/reperfusion, and to explore the related molecular and different interferences mechanism. Meth- ods Wistar rats were randomized into sham groups training groups,ischemia and reperfusion groups and bFGF group. Be- havior training was performed at day 1 after the ischemia and reperfusion. The model of middle cerebral arteries occlusion （MCAO） were performed with intraluminal filament blockade. The expression of N-myc and in the hippocampus was detec- ted with HE and immunohistochemical methods. Results Mter 3 days, N-myc positive cells in the hippocampus were obvi- ously increased. N-myc reaction products were increased gradually with the time of cerebral ischemia/reperfusion, reached the peak on day 14,and then gradually decreased. After training and bFGF intervening, the expression of hippocampal N- myc had apparently increased in each group. Conclusion Behavior training and bFGF promoted the expression of N-myc in the hippocampus tissues. These findings indicate that training and bFGF promotion of neural stem cell proliferation may be mediated by N-myc signaling pathway.

作者邢雪松吕威力吴溪婷

机构地区沈阳医学院解剖学教研室沈阳医学院病理学教研室沈阳医学院信息技术中心计算机教研室

出处《中风与神经疾病杂志》 CAS 北大核心 2015年第7期616-618,共3页 Journal of Apoplexy and Nervous Diseases

基金辽宁省科技厅科学技术计划资助项目(No.2013020216)

关键词行为学训练 N-MYC 神经再生增殖大鼠 Behavior training N-myc Nerve regeneration Proliferation Rat

分类号 R749.13 [医药卫生—神经病学与精神病学]

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