摘要
目的：建立裸鼠体内NB4／ShHMGA2细胞种植瘤模型。探索稳定敲低高迁移率族蛋白A2（HMGA2）的表达对白血病细胞生物学形状的影响。方法：每组5只4。5周龄的BALB／c裸鼠．经4Gy60C0照射后腋窝皮下分别接种8×10^6个NB4／ShHMGA2、NB4／ShControl细胞（对照组），观察成瘤性。3周后处死裸鼠。免疫组化法检测NB4细胞种植瘤组织切片中Ki．67蛋白的表达水平。结果：NB4／shHMGA2及对照细胞在裸鼠腋下均成瘤，NB4／ShHMGA2细胞肿瘤生长速度明显慢于对照组，NB4／ShHMGA2细胞与对照组瘤体体积分别为（1484．25±156．342）和（3228．674±285．64）mm^3，差异有统计学意义（P〈0．05）：NB4／ShHMGA2细胞与对照组瘤块重量分别为（650．46±85．12）和（2135．33±198．05）mg，差异有统计学意义（P〈0．05）。免疫组化检测显示NB4／ShHMGA2细胞组种植瘤组织切片Ki-67蛋白表达水平低于对照组。结论：敲低HMGA2的表达在体内可抑制白血病细胞的增殖。
Objective To establish a nude mice model for NB4/ShHMGA2 xenograft and explore the effect of HMGA2 knockdown on hematological malignancies. Methods NB4/ShHMGA2 or NB4/ShControl cell lines were established by transfecting the recombinant Lentivirus-HMGA2shRNA and the vacant Lentivirus-NC-marked into NB4 cells. The knockdown of HMGA2 was identified by RT-PCR and Western blot. Ten male BALB/c nude mice aged 4 ~ 5 weeks were equally divided into two groups. The mice irradiated by 4 Gy 60 Co were subcutaneously injected with 8 × 10^6 NB4/ShHMGA2 or NB4/ShControl cells into one side of axilla. The volumes of xenograft tumor were evaluated using the equation volume （mm^3） = （L × W2）/2. The xenograft tumor section was detected by IHC with Ki-67 antibody. Results NB4 cell xenograft tumors developed in all mice of both the two groups. The NB4/ShHMGA2 cells in the nude mice grew at a lower rate than those in the controls. There were statistically significant differences in the volume and weight of xenograft tumor between the two groups [ （ 1 484.25 ±156.342）mm^3 vs （3 228.674 ± 285.64）mm^3, P 〈 0.05] and [（2 135.33 ± 198.05） mg vs （650.46 ±85.12）mg, P 〈 0.05]. The Ki-67 protein level in NB4/ShHMGA2 cells xenografts was lower than that in the controls. Conclusion The knockdown of HMGA2 could inhibit proliferation of NB4 cells in NB4 cells xenograft tumor.
出处
《实用医学杂志》
CAS
北大核心
2015年第15期2437-2439,共3页
The Journal of Practical Medicine
基金
广东省科技厅项目(编号:2013B22000102)
广东省医学科研基金项目(编号:A2014292)
