摘要
目的:建立毛细管电泳法(CE)法同时测定复方磷酸可待因口服制剂中磷酸可待因、盐酸麻黄碱、马来酸溴苯那敏、马来酸氯苯那敏、愈创甘油醚等有效成分的含量。方法:色谱柱为贝克曼e CAPTM未涂层石英毛细管柱(50 cm×75μm);分离电压为10 k V;缓冲溶液为20 mmol·L^-1磷酸盐缓冲液(p H 7.5);检测波长为214 nm。结果:各测定组分在考察浓度范围内线性关系良好(r≥0.995),回收率均≥96%。结论:该方法可用于不同复方磷酸可待因口服制剂中有效成分的含量测定,简单有效,具有通用性,便于快速有效的市场监督。
Objective： To establish a method for the simultaneous determination of the active ingredients （ codeine phosphate, brompheniramine maleate, chlorpheniramine maleate, ephedrine hydrochloride and guaifenesin） in compound codeine phosphate oral prelrations by capillary electrophoresis （CE）. Methods： The method employed an uncoated capillary column （ eCAPTM ） from Beck- mann company （50 cm x 75μm） ; the electrophoresis voltage was at 10 kV; 20 mmol · L-1 phosphate buffer solution （pH 7.5 ） was used; the UV measurement was at the wavelength of 214 nm. Results： The studied components had good linear ranges （r≥0. 995 ） within the range of the investigated concentrations. The recovery was no less than 96%. Conclusion： The presented method can be ap- plied in the content determination of active ingredients in compound codeine phosphate oral preparations from different enterprises. It is simple, efficient and universal, which facilitates the market supervision in a fast and valid manner.
出处
《中国药师》
CAS
2015年第8期1312-1315,共4页
China Pharmacist
关键词
毛细管电泳
复方磷酸可待因口服制剂
含量测定
Capillary electrophoresis
Compound codeine phosphate oral preparations
Determination
