摘要
分别以大肠杆菌、金黄色葡萄球菌、沙门氏菌标准株为样本,提取其DNA,以大肠杆菌的pho A基因、金黄色葡萄球菌的nuc A基因和沙门氏菌的inv A基因作为靶基因,建立可以同时检测这3种菌株的多重PCR方法。与国标法对照,探讨多重PCR技术检测乳品样品中3种细菌的灵敏性和特异性。结果表明,建立的多重PCR检测方法与国标法检测的结果一致,人工模拟试验结果稳定,多重PCR具有快速、敏感和特异性强的特点。
The genome DNAs of Escherichia coli, Staphylococcus aureus and Salmonella were extracted. The pho A gene of Escherichia coli, nuc A gene of Staphylococcus and inv A gene of Salmonella were used as the target genes. A multiplex polymerase chain reaction assay for simultaneous identification of three pathogenic bacteria was estab-lished. In contrast to national standard methods, the sensitivity and specificity of multiplex PCR in detecting three bacteria from dairy products were studied. The results showed that the detection results by multiplex PCR method were the same as national standard methods. The results were stable in simulated examination. The multiplex PCR method was more rapid, sensitive and specific than national standard method. The established multiplex PCR method could provide the scientific evidence in detection of pathogenic bacteria from dairy products.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第7期149-154,共6页
Food and Fermentation Industries
基金
国家自然科学基金面上项目(31270479)
