摘要
目的：以金丝桃苷为指标成分,建立刺五加叶的薄层定性和含量测定的方法。方法：采用薄层色谱法对刺五加叶中金丝桃苷进行定性鉴别,并用高效液相色谱法进行含量测定,色谱柱：Kromasil 100-5C18色谱柱（4.6mm×250mm）;流动相：甲醇-0.1%冰醋酸水溶液（35：65）;流速：0.8m L/min;检测波长：360nm;柱温：30℃;进样量：10μL。结果：刺五加叶金丝桃苷的薄层鉴别特征明显,金丝桃苷在0.13~0.78μg范围内具有良好线性关系,r=0.9994,平均回收率为98.17%,RSD为1.02%（n=5）。结论：此方法简便、快速、准确,可用于刺五加叶的质量控制。
Objective： To identify and determine the the leaves ofAcanthopanax senticosus by taking hyperoside as the index composition. Methods：Thin layer chromatography（TLC） was used for the identification and high-performance liquid chromatography （HPLC） was adopted for the determination. Chromatographic column：Kromasil 100-5C18 column （4.6mm× 250mm）;mobile phase： methanol- 0.1%glacial acetic acid（35：65）; flow rate：0.8mL/min;detection wavelength：365nm; sample quantity：lO gL; column tempera- ture：30~C. Results：The characteristic of TLC was distinct. The linear relation of hyperoside was good in the range from 0.13 to 0.78g g. r=-0.9994.Average recovery was 98.17%. RSD was 1.02% （n=5）. Conclusion： This method is sample,rapid,accurate in operation and good in repeat. It is advisable very much for the identification and determination of hyperoside in the leaves of Acanthopanax senticosus.
出处
《黑龙江医药》
CAS
2015年第4期703-705,共3页
Heilongjiang Medicine journal
关键词
刺五加叶
金丝桃苷
薄层色谱法
高效液相色谱法
The leaves of Acanthopanax senticosus
Hyperoside
Thin layer chromatography
High-performance liquid chromatography
