摘要
目的:观察复方中药脑尔康及其拆方组对阿尔茨海默病(AD)模型小鼠皮层、海马中突触素(SYN),高级糖基化终产物受体(RAGE)的影响。方法:随机将70只昆明小鼠分为7组,每组10只,采用ip氯化铝(AlCl3)建立AD模型小鼠,学习记忆成绩测试后,空白组和模型组每天给予生理盐水灌胃;全方组及各拆方组每天分别给予相应药物灌胃(14.3mL·d-1),连续30d。测定各组小鼠皮层和海马SYN、RAGE表达水平。结果:各用药组与模型组比较,全方组及拆方1号组小鼠脑内SYN表达明显增多(P<0.01),各拆方组与全方组比较,拆方1号组与全方组SYN表达无明显差异;各用药组RAGE表达有不同程度的下降(P<0.01或P<0.05),以全方组及拆方1号组效果最为明显,且二者之间拮抗RAGE表达的结果无明显差异,并优于其他用药组。结论:1拆方1号组及脑尔康全方组促进AD模型小鼠脑内SYN表达的作用明显优于其他拆方组;拆方1号组及脑尔康全方组抑制AD模型小鼠脑内RAGE表达的作用明显优于其他拆方组;拆方1号组与全方组治疗作用无明显差异,且药味精简。
Objective ：To observe the amelioration of Naoerkang and its splited prescription on the expression of SYN and RAGE in cortex and hippocampus of senile dementia mice model .Method ：Seventy male Kunming mice were randomly divided into 7 groups with 10 for each group ,then ip AlCl3 solution 100 mg · kg - 1 ,ip ,q .o .d for 40 days to induce the model .After the examination of their ability in learning and memory ,each mouse of the control group and model group had been forced to drink saline solution （0 .5 mL · d - 1 ） ,the other groups had been forced to drink the re‐sponse medicine （14 .3 g · kg - 1 · d - 1 ） every day for 30 days .The positive expression of SYN and RAGE in right cortex , hippocampus ,and the dentate gyrus of the mice in each group were examined .Result ：Compared with the model group , the expression of SYN was increased evidently （ P 〈 0 .01）in the mice of Naoerkang group and splited prescription 1 group ,while the expression of different levels increased only in other groups （P〈 0 .05） .The expression of RAGE was decreased evidently （P 〈 0 .01 or P 〈 0 .05） in the mice of Naoerkang group and all of its decomposed recipes .There were no significant differences existed in splited prescription 1 and Naoerkang group .Conclusion ：Decomposed recipe 1 and Naoerkang groups have a better effect of promoting the expression of SYN in brains of AD mice model than any other splited prescription .Decomposed recipe 1 and Naoerkang groups have a better effect of inhibiting the expression of RAGE in brains of AD mice model than any other splited prescription .There were no significant differences between the thera‐peutic effect in splited prescription 1 and Naoerkang group .
出处
《陕西中医》
2015年第8期1078-1080,共3页
Shaanxi Journal of Traditional Chinese Medicine
基金
陕西省科技攻关计划项目(2005K10-G12)
关键词
阿尔茨海默病/中医药疗法
脑尔康
动物实验
小鼠
Alzheimer disease/ traditional Chinese medicine therapy
Naoerkang
Animal experimen-tation
Mice
