摘要
目的 建立大鼠跖骨模型,观察氟对跖骨纵向生长的影响及其病理学改变.方法 选取新生24 h内SD大鼠24只,采用随机数字表法分为4组,每组6只.取大鼠第2、3、4跖骨进行器官培养,采用左后跖骨和右后跖骨自身配对的方法,右侧跖骨染氟,左侧跖骨作为对照,4组大鼠右侧跖骨分别用含1＆#215;10-7、1＆#215;10-6、1＆#215;10-5、1×10^-4 mol/L氟离子的培养液培养,左侧跖骨给予同等体积无氟离子的培养液,在培养的0、1、4、7d时分别测量跖骨的矿化区长度、宽度并计算跖骨纵向生长率,根据软骨细胞在不同部位的特点测量增殖层和肥大层的厚度.培养7d后行常规包埋切片,光镜下观察氟对跖骨病理学的影响.结果 培养7d后,1×10^-4mol/L组的大鼠右侧跖骨矿化区长度的增长[（240.5±139.3） μm]显著低于其左侧跖骨[（394.1±173.9）μm,t=4.37,P＜0.01];1×10^-4 mol/L组右侧跖骨[（239.9±119.4）μm]与左侧跖骨[（223.3±99.9）μm]的跖骨矿化区宽度增长比较,差异无统计学意义（t=0.44,P＞0.05）.在培养4、7d时,1×10^-4 mol/L组的右侧跖骨纵向生长率[（2.43±144）％、（16.16±2.87）％]低于左侧跖骨[（8.34±1.74）％、（26.52±4.46）％,t=3.21、3.13,P均＜0.01].甲苯胺蓝染色显示,1×10^-4 mol/L组右侧跖骨增殖层及肥大层软骨细胞厚度[（111.33±27.29）、（125.33±30.08）μm]都显著低于左侧跖骨[（127.33±38.36）、（160.50±42.73）μm,t=4.82、5.81,P均＜0.01];1×10^-4 mol/L组右侧跖骨增殖层厚度/肥大层厚度比值（0.93±0.36）明显高于左侧跖骨（0.83±0.32,t=4.42,P＜0.01）.结论 过量氟抑制大鼠跖骨的纵向生长,表现为跖骨纵向生长率降低;染氟组跖骨增殖层和肥大层均减少、增殖层与肥大层的比例失衡.
Objective To study the effects of fluoride on longitudinal growth and pathological changes of cultured rat metatarsal bone rudiments.Methods Twenty-four neonatal SD rats were divided into four groups according to the random number table,then the second,third and fourth metatarsal bone rudiments were surgically isolated.The left metatarsal bone rudiments were cultured in α-MEM without F-as control group and the right metatarsal bone rudiments from the same rat were cultured in α-MEM with 1 ＆#215; 10-7,1 ＆#215; 10-6,1 ＆#215; 10-5 and 1 ×10^-4 mol/L F-.The length and width of the mineralized area of metatarsal were measured on day 0,day 1,day 4 and day 7,respectively,and the pathological changes of metatarsal bone rudiments were observed by the routinely paraffin-embedded sections method on day 7.Results On day 7,the length of the mineralized area was significantly lower of right metatarsal bone [（240.5 ± 139.3）μm] than the left metatarsal bone [（394.1 ± 173.9）μm,t =4.37,P 〈 0.01] in the 1 ×10^-4 mol/L F-group,but the width of the mineralized area [（239.9 ± 119.4）μm] was not different significantly compared to the left metatarsal bone [（223.3 ± 99.9）μm,t =0.44,P 〉 0.05].The relative vertical growth rate of the mineralized area on day 4 was significantly lower of right metatarsal bone [（2.43 ± 1.44）%] than left metatarsal bone [（8.34 ± 1.74）%,t =3.21,P 〈 0.01] in 1 ×10^-4 mol/L F-group,and the difference was also significant on day 7 [（16.16 ± 2.87）% vs.（26.52 ± 4.46）%,t =3.13,P 〈 0.01].Toluidine blue staining results showed that the thickness of cartilage cells of proliferation and hypertrophic layers was significantly lower of right metatarsal bone [（111.33 ± 27.29）,（125.33 ± 30.08）μm] than left metatarsal bone [（127.33 ± 38.36）,（160.50 ± 42.73）μm,t =4.82,5.81,all P 〈 0.01] in 1 ×10^-4 mol/L F-group.The ratio of proliferative and hypertrophic layers was significantly higher of right metatarsal bone （0.93 ± 0.36） than left metatarsal bone （0.83 ± 0.32,t =4.42,P 〈 0.01） in 1 ×10^-4 mol/L F-group.Conclusions Our findings indicate that excessive fluoride could cause longitudinal bone growth inhibition.Such growth inhibition is mediated by decreased chondrocyte proliferation and differentiation and the disproportion of proliferation and differentiation.
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2015年第8期564-568,共5页
Chinese Journal of Endemiology
基金
辽宁省科学技术计划项目（2013225049）
关键词
氟中毒
跖骨
软骨
关节
Fluorosis
Metatarsal bone
Cartilage,articular
