摘要
目的建立测定骨痛药酒中大黄素含量的高效液相色谱法。方法色谱柱：XBridgeTMShield RP18柱（4.6×250 mm,5μm）;流动相：甲醇-0.1%磷酸溶液（80：20）;流速：1.0 m L·min-1;柱温：30℃;检测波长：254 nm。结果大黄素在3.26~90.60μg·ml-1范围内与面积线性关系良好,r=0.999 9,平均回收率为98.58%,RSD 1.30%（n=6）。结论本法操作简单,可靠,重复性好,适用于骨痛药酒的质量控制。
Objective To establish an HPLC method for determining the Emodin content in Gutong medicated wine. Methods The XBridgeTM Shield RP18 column （4.6 × 250 mm,5 μm）was used with the mobile phase methanol -0. 1% phosphoric acid solution （80： 20） ;The flow rate was 1. 0mL. min-land the column temperature was maintained at 30 ℃ ; The UV detection wavelength was set at 254 nm. Results Emodin showed good linear relationship in the range of 3.26 - 90. 60 μg.ml-1 （ r = 0. 999 9） , the average recovery was 98.58% , RSD was 1.30% （n = 6）. Conclusion The method is accurate, easy and reproducible, and suitable for the quality control of Gutong medicated wine,which can be used for quality control of Gutong medicated wine.
出处
《光明中医》
2015年第8期1641-1642,共2页
GUANGMING JOURNAL OF CHINESE MEDICINE
